Cathepsin L/V/K/H Antibody (JM10-78)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-67216
Recombinant Monoclonal Antibody.
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Mouse
Applications
Validated:
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # JM10-78
Concentration
1 mg/ml
Product Specifications
Immunogen
Synthetic peptide within Human Cathepsin L/V/K/H aa 92-138 / 333. (SwissProt: P07711 Human; SwissProt: O60911 Human; SwissProt: P43235 Human; SwissProt: P09668 Human)
Localization
Cell membrane, Cytoplasmic vesicle, Lysosome, Membrane, Nucleus, Secreted.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Cathepsin L/V/K/H Antibody (JM10-78)
Western Blot: Cathepsin L/V/K/H Antibody (JM10-78) [NBP2-67216]
Western Blot: Cathepsin L/V/K/H Antibody (JM10-78) [NBP2-67216] - Western blot analysis of Cathepsin L/V/K/H on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HepG2 cell lysate Lane 2: A549 cell lysateImmunohistochemistry-Paraffin: Cathepsin L/V/K/H Antibody (JM10-78) [NBP2-67216]
Immunohistochemistry-Paraffin: Cathepsin L/V/K/H Antibody (JM10-78) [NBP2-67216] - Analysis of paraffin-embedded rat kidney tissue using anti-Cathepsin L/V/K/H antibody. Counter stained with hematoxylin.Flow Cytometry: Cathepsin L/V/K/H Antibody (JM10-78) [NBP2-67216]
Flow Cytometry: Cathepsin L/V/K/H Antibody (JM10-78) [NBP2-67216] - Analysis of HepG2 cells with Cathepsin L/V/K/H antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.Applications for Cathepsin L/V/K/H Antibody (JM10-78)
Application
Recommended Usage
Flow Cytometry
1:50-1:100
Immunocytochemistry/ Immunofluorescence
1:50-1:100
Immunohistochemistry-Paraffin
1:50-1:200
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
TBS (pH7.4), 0.05% BSA, 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Cathepsin L/V/K/H
Alternate Names
ACC4, ACC-4, ACC5, ACC-5, aleurain, cathepsin B3, cathepsin BA, cathepsin K, cathepsin L1, cathepsin L2, cathepsin L2, preproprotein, Cathepsin O, cathepsin O1, Cathepsin O2, Cathepsin U, Cathepsin X, CATL, CATL2, CPSB, CTS02, CTSL1, CTSL2, CTSO, CTSO1, CTSO2, CTSU, Major excreted protein, MEP, N-benzoylarginine-beta-naphthylamide hydrolase, PKND, procathepsin L, PYCD
Gene Symbol
CTSL
Additional Cathepsin L/V/K/H Products
Product Documents for Cathepsin L/V/K/H Antibody (JM10-78)
Product Specific Notices for Cathepsin L/V/K/H Antibody (JM10-78)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...
Loading...