Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Hamster, Rabbit

Cited:

Human, Mouse, Rat

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Microarray, Western Blot

Cited:

IF/IHC, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # S57

Concentration

1 mg/ml

View all Cav1.2 Antibodies »

Product Specifications

Immunogen

Fusion protein amino acids 1507-1733 (intracellular carboxyl terminus) of rabbit Cav1.2

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 22038741).

Localization

Membrane, Cell Membrane

Specificity

Detects approx 240 kDa (varies with cell background due to glycosylation).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Cav1.2 Antibody (S57)

Western Blot: Cav1.2 Antibody (S57) [NBP1-22439]

Western Blot: Cav1.2 Antibody (S57) [NBP1-22439]

Western Blot: Cav1.2 Antibody (S57) [NBP1-22439] - Western Blot analysis of Hamster T-CHO cell lysate showing detection of CaV1.2 Calcium Channel protein using Mouse Anti-CaV1.2 Calcium Channel Monoclonal Antibody, Clone S57 (NBP1-22439). Primary Antibody: Mouse Anti-CaV1.2 Calcium Channel Monoclonal Antibody (NBP1-22439) at 1:1000.
Immunocytochemistry/ Immunofluorescence: Cav1.2 Antibody (S57) [NBP1-22439]

Immunocytochemistry/ Immunofluorescence: Cav1.2 Antibody (S57) [NBP1-22439]

Immunocytochemistry/Immunofluorescence: Cav1.2 Antibody (S57) [NBP1-22439] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Cav1.2 Monoclonal Antibody, Clone S57 (NBP1-22439). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Cav1.2 Monoclonal Antibody (NBP1-22439) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Cav1.2 Antibody (D) Composite.
Immunohistochemistry: Cav1.2 Antibody (S57) [NBP1-22439]

Immunohistochemistry: Cav1.2 Antibody (S57) [NBP1-22439]

Immunohistochemistry: Cav1.2 Antibody (S57) [NBP1-22439] - Immunohistochemistry analysis using Mouse Anti-CaV1.2 Calcium channel Monoclonal Antibody, Clone S57 (NBP1-22439). Tissue: Brain Tissue. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-CaV1.2 Calcium channel Monoclonal Antibody (NBP1-22439) at 1:10000 for 12 hours at 4C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 l for 2 minutes at RT. Magnification: 40x.
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Applications for Cav1.2 Antibody (S57)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:1000

Immunohistochemistry-Paraffin

0.1 - 1.0 ug/mL

Immunoprecipitation

1:200

Western Blot

1:1000
Application Notes
1 ug/mL of Cav1.2 Antibody was sufficient for detection of Cav1.2 in 10 ug of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 50% Glycerol

Preservative

0.1% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Cav1.2

Ion channels are integral membrane proteins that help establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient (1). They are present in the membranes that surround all biological cells because their main function is to regulate the flow of ions across this membrane. Whereas some ion channels permit the passage of ions based on charge, others conduct based on an ionic species, such as sodium or potassium. Furthermore, in some ion channels, the passage is governed by a gate which is controlled by chemical or electrical signals, temperature, or mechanical forces. There are a few main classifications of gated ion channels. There are voltage- gated ion channels, ligandgated, other gating systems and finally those that are classified differently, having more exotic characteristics. The first are voltage- gated ion channels which open and close in response to membrane potential. These are then separated into sodium, calcium, potassium, proton, transient receptor, and cyclic nucleotide-gated channels; each of which is responsible for a unique role. Ligand-gated ion channels are also known as ionotropic receptors, and they open in response to specific ligand molecules binding to the extracellular domain of the receptor protein. The other gated classifications include activation and inactivation by second messengers, inward-rectifier potassium channels, calcium-activated potassium channels, two-pore-domain potassium channels, light-gated channels, mechano-sensitive ion channels and cyclic nucleotide-gated channels. Finally, the other classifications are based on less normal characteristics such as two-pore channels, and transient receptor potential channels (2). Specifically, Cav1.2 is a cardiac L-type calcium channel, and is important for excitation and contraction of the heart (3). It may be associated with a variant of Long QT syndrome called Timothy's syndrome (4, 5) and also with Brugada syndrome. Some references also suggest it is related to bipolar disease as well (5).

Long Name

Voltage-dependent L-type calcium channel subunit alpha-1C

Alternate Names

CACH2, CACN2, CACNA1C, CACNL1A1, CCHL1A1

Entrez Gene IDs

775 (Human); 12288 (Mouse); 24239 (Rat); 100101555 (Rabbit)

Gene Symbol

CACNA1C

UniProt

P15381 (Rabbit)

Additional Cav1.2 Products

Product Documents for Cav1.2 Antibody (S57)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Cav1.2 Antibody (S57)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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