Caveolin-1 Antibody (M281)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23193
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Chicken
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # M281
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for Caveolin-1 Antibody (M281)
Immunogen
Clone (M281) was generated from a full length recombinant human Caveolin-1 protein. [UniProt# Q03135]
Specificity
This antibody detects a 21 kDa protein corresponding to the apparent molecular mass of caveolin-1 on SDS-PAGE immunoblots of human A431 cells and rabbit spleen fibroblasts.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Stable for at least 1 year at -20°C.
Scientific Data Images for Caveolin-1 Antibody (M281)
Western Blot: Caveolin-1 Antibody (M281) [NBP3-23193] -
Western Blot: Caveolin-1 Antibody (M281) [NBP3-23193] - Western blot image of human A431 cells unstimulated (lanes 1, 3, & 5) or stimulated with pervanadate (1 mM) for 30 min (lanes 2, 4, & 6). The blots were probed with rabbit polyclonal caveolin-1 (N-term.) (lanes 1 & 2), mouse monoclonal caveolin-1 (Tyr-14) (lanes 3 & 4) or mouse monoclonal caveolin-1 (lanes 5 & 6).
Immunocytochemistry/Immunofluorescence: Caveolin-1 Antibody (M281) [NBP3-23193] -
Immunocytochemistry/Immunofluorescence: Caveolin-1 Antibody (M281) [NBP3-23193] - Immunocytochemical labeling of caveolin-1 in paraformaldehyde-fixed and NP-40-permeabilized rabbit spleen fibroblasts. The cells were labeled with rabbit polyclonal Caveolin-1 (N-terminal region) and mouse monoclonal Caveolin-1 antibodies, and detected using appropriate secondary antibodies conjugated to Cy3. Phase contrast images (left) and immunofluorescent images (right).Applications for Caveolin-1 Antibody (M281)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50
Western Blot
1:500
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS, 1 mg/ml BSA and 50% glycerol
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C.
Background: Caveolin-1
Alternate Names
CAV1, Caveolin1, MSTP085, VIP21
Gene Symbol
CAV1
Additional Caveolin-1 Products
Product Documents for Caveolin-1 Antibody (M281)
Product Specific Notices for Caveolin-1 Antibody (M281)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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