CBFB Antibody (PCRP-CBFB-1E6)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13808

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Protein Array, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # PCRP-CBFB-1E6

Concentration

0.2 mg/ml

View all CBFB Antibodies »

Product Specifications

Immunogen

Recombinant full-length human CBFB protein (Uniprot: Q13951)

Localization

Nucleus, Nucleoplasm, Cytoplasm

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

22 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14059)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for CBFB Antibody (PCRP-CBFB-1E6)

Western Blot: CBFB Antibody (PCRP-CBFB-1E6) [NBP3-13808]

Western Blot: CBFB Antibody (PCRP-CBFB-1E6) [NBP3-13808]

Western Blot: CBFB Antibody (PCRP-CBFB-1E6) [NBP3-13808] - Western blot analysis of HeLa cell lysate using CBFB antibody (PCRP-CBFB-1E6).
Immunocytochemistry/ Immunofluorescence: CBFB Antibody (PCRP-CBFB-1E6) [NBP3-13808]

Immunocytochemistry/ Immunofluorescence: CBFB Antibody (PCRP-CBFB-1E6) [NBP3-13808]

Immunocytochemistry/Immunofluorescence: CBFB Antibody (PCRP-CBFB-1E6) [NBP3-13808] - Immunofluorescence Analysis of PFA-fixed U87 cells stained using CBFB Antibody (PCRP-CBFB-1E6) followed by goat anti-mouse IgG-CF488 (green). CF640R phalloidin (red).
Flow Cytometry: CBFB Antibody (PCRP-CBFB-1E6) [NBP3-13808]

Flow Cytometry: CBFB Antibody (PCRP-CBFB-1E6) [NBP3-13808]

Flow Cytometry: CBFB Antibody (PCRP-CBFB-1E6) [NBP3-13808] - Flow cytometric analysis of PFA-fixed HeLa cells. CBFB antibody (PCRP-CBFB-1E6) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).
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Applications for CBFB Antibody (PCRP-CBFB-1E6)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunoprecipitation

1-2 ug/100-500 ug protein

Western Blot

1-2 ug/ml
Application Notes
ELISA: For coating, order antibody without BSA

Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate)

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: CBFB

The transcription factor Polyomavirus enhancer binding protein 2 (PEBP2), also designated Osf2 (Osteoblast-specific transcription factor), CBFA1 (Core Binding Factor) and AML3 (Acute myeloid leukemia), is composed of two subunits, alpha and beta, which are essential for the regulation of hematopoiesis and osteogenesis. The PEBP2alpha subunits, PEBP2alphaA, PEBP2alphaB and PEBP2alphaC, are encoded by three RUNX genes, all of which contain a 128-amino acid region homologous to the highly conserved Drosophila segmentation gene, runt. This region is involved in DNA binding and heterodimerization with the regulatory beta subunit, which facilitates DNA binding of the alpha subunit. Both subunits are required for in vivo function; the disruption of either gene results in a lack of definitive hematopoiesis followed by embryo death in utero due to hemorrhage in the central nervous system. The gene encoding PEBP2beta is the target of chromosomal inversion 16 (p13;q22) with the smooth muscle myosin heavy chain, producing a chimeric gene, PEBP2beta/CBFbeta-SMMHC, that is associated with human acute myeloid leukemia.

Long Name

Core-binding Factor Subunit beta

Alternate Names

CBF-beta, PEA2-beta, PEBP2B, SL3-3

Gene Symbol

CBFB

UniProt

Q13951 (Human)

Additional CBFB Products

Product Documents for CBFB Antibody (PCRP-CBFB-1E6)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CBFB Antibody (PCRP-CBFB-1E6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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