CBLB Antibody (PSH03-49)

Western Blot: CBLB Antibody (PSH03-49) [NBP3-32113]

Western Blot: CBLB Antibody (PSH03-49) [NBP3-32113] - Western blot analysis of CBLB on different lysates with Rabbit anti-CBLB antibody (NBP3-32113) at 1/2,000 dilution.

Lane 1: Jurkat cell lysate (20 ug/Lane)
Lane 2: K-562 cell lysate (20 ug/Lane)
Lane 3: U-87 MG cell lysate (20 ug/Lane)
Lane 4: HeLa cell lysate (20 ug/Lane)
Lane 5: A431 cell lysate (20 ug/Lane)
Lane 6: A549 cell lysate (20 ug/Lane)
Lane 7: NIH/3T3 cell lysate (20 ug/Lane)
Lane 8: RAW264.7 cell lysate (20 ug/Lane)
Lane 9: PC-12 cell lysate (20 ug/Lane)
Lane 10: Mouse lung tissue lysate (40 ug/Lane)

Predicted band size: 109 kDa
Observed band size: 109 kDa

Exposure time: 24 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32113) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: CBLB Antibody (PSH03-49) [NBP3-32113]

Immunohistochemistry: CBLB Antibody (PSH03-49) [NBP3-32113] - Immunohistochemical analysis of paraffin-embedded human stomach tissue with Rabbit anti-CBLB antibody (NBP3-32113) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32113) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.