Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all CBP20 Antibodies »

Product Specifications

Immunogen

The immunogen for this product maps to a region between residue 106 to 156 of human nuclear cap binding protein subunit 2 using the numbering given in entry NP_031388.2 (GeneID 22916).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

18 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CBP20 Antibody

Western Blot: CBP20 Antibody [NBP1-30445]

Western Blot: CBP20 Antibody [NBP1-30445]

Western Blot: CBP20 Antibody [NBP1-30445] - Detection of Human and Mouse NCBP2 Whole cell lysate from HeLa and mouse NIH3T3 cells. NCBP2 was also immunoprecipitated by rabbit anti-NCBP2 antibody NBP1-30444.
Immunohistochemistry-Paraffin: CBP20 Antibody [NBP1-30445]

Immunohistochemistry-Paraffin: CBP20 Antibody [NBP1-30445]

Immunohistochemistry-Paraffin: CBP20 Antibody [NBP1-30445] - Sample: FFPE section of human breast carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti- NCBP2 used at a dilution of 1:5,000 (0.2ug/ml) and 1:1,000 (1 ug/ml). Detection: DAB

Applications for CBP20 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:1000 - 1:5000

Immunoprecipitation

5-15 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CBP20

The product of this gene is a component of the nuclear cap-binding protein complex (CBC), which binds to the monomethylated 5' cap of nascent pre-mRNA in the nucleoplasm. The encoded protein has an RNP domain commonly found in RNA binding proteins, and contains the cap-binding activity. The CBC promotes pre-mRNA splicing, 3'-end processing, RNA nuclear export, and nonsense-mediated mRNA decay. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Alternate Names

20 kDa nuclear cap-binding protein, Cbc2, CBP20CBC2, NCBP 20 kDa subunit, NCBP interacting protein 1, NCBP-interacting protein 1, NIP1Cell proliferation-inducing gene 55 protein, nuclear cap binding protein subunit 2, 20kD, nuclear cap binding protein subunit 2, 20kDa, nuclear cap-binding protein subunit 2

Entrez Gene IDs

22916 (Human)

Gene Symbol

NCBP2

UniProt

P52298 (Human)

Additional CBP20 Products

Product Documents for CBP20 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CBP20 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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