CBX1 Antibody (4E12)

Novus Biologicals, part of Bio-Techne | Catalog # H00010951-M01

Novus Biologicals, part of Bio-Techne
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H00010951-M01
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 4E12

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all CBX1 Antibodies »

Product Specifications

Immunogen

CBX1 (NP_006798.1, 2 a.a. ~ 99 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. GKKQNKKKVEEVLEEEEEEYVVEKVLDRRVVKGKVEYLLKWKGFSDEDNTWEPEENLDCPDLIAEFLQSQKTAHETDKSEGGKRKADSDSEDKGEESK

Specificity

CBX1 - chromobox homolog 1 (HP1 beta homolog Drosophila ) (4E12)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for CBX1 Antibody (4E12)

Western Blot: CBX1 Antibody (4E12) [H00010951-M01]

Western Blot: CBX1 Antibody (4E12) [H00010951-M01]

Western Blot: CBX1 Antibody (4E12) [H00010951-M01] - Analysis of CBX1 expression in transfected 293T cell line by CBX1 monoclonal antibody (M01), clone 4E12.Lane 1: CBX1 transfected lysate(21.4 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: CBX1 Antibody (4E12) [H00010951-M01]

Immunocytochemistry/ Immunofluorescence: CBX1 Antibody (4E12) [H00010951-M01]

Immunocytochemistry/Immunofluorescence: CBX1 Antibody (4E12) [H00010951-M01] - Analysis of monoclonal antibody to CBX1 on HeLa cell . Antibody concentration 10 ug/ml.
Sandwich ELISA: CBX1 Antibody (4E12) [H00010951-M01] - Detection limit for recombinant GST tagged CBX1 is 0.1 ng/ml as a capture antibody.

Applications for CBX1 Antibody (4E12)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody Reactive Against Recombinant Protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: CBX1

This gene encodes a highly conserved nonhistone protein, which is a member of the heterochromatin protein family . The protein is enriched in the heterochromatin and associated with centromeres. The protein has a single N-terminal chromodomain which can bind to histone proteins via methylated lysine residues, and a C-terminal chromo shadow-domain (CSD) which is responsible for the homodimerization and interaction with a number of chromatin-associated nonhistone proteins. The protein may play an important role in the epigenetic control of chromatin structure and gene expression. Several related pseudogenes are located on chromosomes 1, 3, and X. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq]

Alternate Names

CBXM31HP1-BETA, chromobox homolog 1, chromobox homolog 1 (Drosophila HP1 beta), chromobox homolog 1 (HP1 beta homolog Drosophila ), chromobox protein homolog 1, Heterochromatin protein 1 homolog beta, heterochromatin protein 1-beta, Heterochromatin protein p25, heterochromatin protein p25 beta, HP1 beta, HP1 beta homolog, HP1Hsbeta, HP1Hs-beta, MOD1, Modifier 1 protein, p25beta

Entrez Gene IDs

10951 (Human)

Gene Symbol

CBX1

UniProt

NP_006798 (Human)

Additional CBX1 Products

Product Documents for CBX1 Antibody (4E12)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for CBX1 Antibody (4E12)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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