CBX3 Antibody (1G12-1D9)

Novus Biologicals, part of Bio-Techne | Catalog # H00011335-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1G12-1D9

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all CBX3 Antibodies »

Product Specifications

Immunogen

CBX3 (AAH00954, 1 a.a. ~ 183 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MASNKTTLQKMGKKQNGKSKKVEEAEPEEFVVEKVLDRRVVNGKVEYFLKWKGFTDADNTWEPEENLDCPELIEAFLNSQKAGKEKDGTKRKSLSDSESDDSKSKKKRDAADKPRGFARGLDPERIIGATDSSGELMLLMKWKDSDEADLVLAKEANMKCPQIVIAFYEERLTWHSCPEDEAQ

Reactivity Notes

Other species not tested.

Specificity

CBX3 - chromobox homolog 3 (HP1 gamma homolog, Drosophila)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for CBX3 Antibody (1G12-1D9)

Western Blot: CBX3 Antibody (1G12-1D9) [H00011335-M01]

Western Blot: CBX3 Antibody (1G12-1D9) [H00011335-M01]

Western Blot: CBX3 Antibody (1G12-1D9) [H00011335-M01] - CBX3 monoclonal antibody (M01), clone 1G12-1D9 Analysis of CBX3 expression in HeLa.
Immunocytochemistry/ Immunofluorescence: CBX3 Antibody (1G12-1D9) [H00011335-M01]

Immunocytochemistry/ Immunofluorescence: CBX3 Antibody (1G12-1D9) [H00011335-M01]

Immunocytochemistry/Immunofluorescence: CBX3 Antibody (1G12-1D9) [H00011335-M01] - Analysis of monoclonal antibody to CBX3 on HeLa cell. Antibody concentration 10 ug/ml.
Sandwich ELISA: CBX3 Antibody (1G12-1D9) [H00011335-M01] - Detection limit for recombinant GST tagged CBX3 is approximately 0.03ng/ml as a capture antibody.

Applications for CBX3 Antibody (1G12-1D9)

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: CBX3

At the nuclear envelope, the nuclear lamina and heterochromatin are adjacent to the inner nuclear membrane. The protein encoded by this gene binds DNA and is a component of heterochromatin. This protein also can bind lamin B receptor, an integral membrane protein found in the inner nuclear membrane. The dual binding functions of the encoded protein may explain the association of heterochromatin with the inner nuclear membrane. Two transcript variants encoding the same protein but differing in the 5' UTR, have been found for this gene.

Alternate Names

chromobox homolog 3, chromobox homolog 3 (Drosophila HP1 gamma), chromobox protein homolog 3, Drosophila), HECH, Heterochromatin protein 1 homolog gamma, heterochromatin protein HP1 gamma, heterochromatin-like protein 1, HP1 gamma, HP1 gamma homolog, HP1-GAMMA, Modifier 2 protein

Entrez Gene IDs

11335 (Human)

Gene Symbol

CBX3

OMIM

604477 (Human)

UniProt

AAH00954 (Human)

Additional CBX3 Products

Product Documents for CBX3 Antibody (1G12-1D9)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CBX3 Antibody (1G12-1D9)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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