CBX3 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-92962

Novus Biologicals, part of Bio-Techne
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NBP2-92962-0.02ml
NBP2-92962-0.1ml
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Knockout Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CBX3 Antibodies »

Product Summary for CBX3 Antibody - BSA Free

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-183 of human CBX3 (NP_009207.2). MASNKTTLQKMGKKQNGKSKKVEEAEPEEFVVEKVLDRRVVNGKVEYFLKWKGFTDADNTWEPEENLDCPELIEAFLNSQKAGKEKDGTKRKSLSDSESDDSKSKKKRDAADKPRGFARGLDPERIIGATDSSGELMFLMKWKDSDEADLVLAKEANMKCPQIVIAFYEERLTWHSCPEDEAQ

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

20 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CBX3 Antibody - BSA Free

Western Blot: CBX3 AntibodyBSA Free [NBP2-92962]

Western Blot: CBX3 AntibodyBSA Free [NBP2-92962]

Western Blot: CBX3 Antibody [NBP2-92962] - Western blot analysis of extracts from normal (control) and HP1 gamma/CBX3 knockout (KO) 293T cells, using HP1 gamma/CBX3 antibody (NBP2-92962) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunocytochemistry/ Immunofluorescence: CBX3 Antibody - BSA Free [NBP2-92962]

Immunocytochemistry/ Immunofluorescence: CBX3 Antibody - BSA Free [NBP2-92962]

Immunocytochemistry/Immunofluorescence: CBX3 Antibody [NBP2-92962] - Immunofluorescence analysis of C6 cells using HP1 gamma/CBX3 antibody (NBP2-92962) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: CBX3 Antibody - BSA Free [NBP2-92962]

Immunohistochemistry-Paraffin: CBX3 Antibody - BSA Free [NBP2-92962]

Immunohistochemistry-Paraffin: CBX3 Antibody [NBP2-92962] - Immunohistochemistry of paraffin-embedded mouse liver using [KO Validated] HP1 gamma/CBX3 Rabbit pAb (NBP2-92962) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
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Applications for CBX3 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Immunoprecipitation

1:500 - 1:1000

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS with 50% glycerol, pH7.3.

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CBX3

HP1 gamma appears to be involved in transcriptional silencing in heterochromatin-like complexes. It recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. HP1 gamma may contribute to the association of the heterochromatin with the inner nuclear membrane through its interaction with lamin B receptor (LBR) and involved in the formation of functional kinetochore through interaction with MIS12 complex proteins.

Alternate Names

chromobox homolog 3, chromobox homolog 3 (Drosophila HP1 gamma), chromobox protein homolog 3, Drosophila), HECH, Heterochromatin protein 1 homolog gamma, heterochromatin protein HP1 gamma, heterochromatin-like protein 1, HP1 gamma, HP1 gamma homolog, HP1-GAMMA, Modifier 2 protein

Gene Symbol

CBX3

Additional CBX3 Products

Product Documents for CBX3 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for CBX3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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