CBX3 [p Ser83] Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-38641
Key Product Details
Species Reactivity
Human, Mouse
Applications
ELISA, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic phosphorylated peptide around S83 of human CBX3 (NP_009207.2).
Sequence:
RKSLS
Sequence:
RKSLS
Modification
p Ser 83
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for CBX3 [p Ser83] Antibody
![CBX3 [p Ser83] Antibody](https://resources.bio-techne.com/images/products/nbp3-38641_rabbit-cbx3-p-ser-83-pab-101220241243937.jpg "Western Blot: CBX3 [p Ser83] Antibody [NBP3-38641] -")
Western Blot: CBX3 [p Ser83] Antibody [NBP3-38641] -
Western Blot: CBX3 [p Ser83] Antibody [NBP3-38641] - Western blot analysis of lysates from NIH/3T3 cells, using CBX3 Rabbit pAb at 1:1000 dilution. NIH/3T3 cells were treated by Paclitaxel (100 nM) at 37C for 20 hours.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 180s.
![CBX3 [p Ser83] Antibody](https://resources.bio-techne.com/images/products/nbp3-38641_rabbit-cbx3-p-ser-83-pab-1012202411572645.jpg "Western Blot: CBX3 [p Ser83] Antibody [NBP3-38641] -")
Western Blot: CBX3 [p Ser83] Antibody [NBP3-38641] -
Western Blot: CBX3 [p Ser83] Antibody [NBP3-38641] - Western blot analysis of lysates from HeLa cells, using CBX3at 1:1000 dilution or HP1 gamma/CBX3 antibody. HeLa cells were treated by Paclitaxel (100 nM/mL) at 37C for 20 hours.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit.
Exposure time: 60s.
Applications for CBX3 [p Ser83] Antibody
Application
Recommended Usage
Western Blot
1:500 - 1:1000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol
Preservative
0.01% Thimerosal
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: CBX3
Alternate Names
chromobox homolog 3, chromobox homolog 3 (Drosophila HP1 gamma), chromobox protein homolog 3, Drosophila), HECH, Heterochromatin protein 1 homolog gamma, heterochromatin protein HP1 gamma, heterochromatin-like protein 1, HP1 gamma, HP1 gamma homolog, HP1-GAMMA, Modifier 2 protein
Gene Symbol
CBX3
Additional CBX3 Products
Product Documents for CBX3 [p Ser83] Antibody
Product Specific Notices for CBX3 [p Ser83] Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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