Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunoprecipitation, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all CCAR1 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 700 and 750 of human Cell-Cycle and Apoptosis Regulatory Protein 1 using the numbering given in entry NP_060707.2 (GeneID 55749).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CCAR1 Antibody

Western Blot: CCAR1 Antibody [NB100-258]

Western Blot: CCAR1 Antibody [NB100-258]

Western Blot: CCAR1 Antibody [NB100-258] - Detection of Human CCAR1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CCAR1 antibody NB100-258 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Western Blot: CCAR1 Antibody [NB100-258]

Western Blot: CCAR1 Antibody [NB100-258]

Western Blot: CCAR1 Antibody [NB100-258] - Detection of Human DIS on HeLa whole cell lystate using NB100-258. Controls: Normal IgG or another rabbit anti-DIS.

Applications for CCAR1 Antibody

Application
Recommended Usage

Immunoprecipitation

1-4 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
By Western blot, this antibody recognizes bands around 150 kDa representing DIS. Also useful for Immunoprecipitation.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CCAR1

CCAR1 was identified as a perinuclear phosphoprotein that regulates apoptosis signals in response to chemotherapeutic agents. Induction of apoptosis by CCAR1 involves the epidermal growth factor receptor (EGFR), mitogen-activated protein kinase pathway and caspase-9 activation. CARP-1 has also been demonstrated as a suppressor of human breast cancer growth. CCAR1 is also known as cell division cycle and apoptosis regulator protein 1, cell cycle and apoptosis regulatory protein 1, CARP-1, death inducer with SAP domain, DIS, MGC44628, and RP11-437A18.1.

Alternate Names

CARP-1, CARP1MGC44628, Cell cycle and apoptosis regulatory protein 1, cell division cycle and apoptosis regulator 1, cell division cycle and apoptosis regulator protein 1, Death inducer with SAP domain, DIS, FLJ10590, RP11-437A18.1

Entrez Gene IDs

55749 (Human)

Gene Symbol

CCAR1

UniProt

NP_060707.2 (Human)

Additional CCAR1 Products

Product Documents for CCAR1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for CCAR1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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