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CCBE1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-79501

Novus Biologicals, part of Bio-Techne
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NBP1-79501

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.5 mg/ml

Product Specifications

Immunogen

Synthetic peptide directed towards the C terminal of human Ccbe1The immunogen for this antibody is Ccbe1. Peptide sequence RGAPGPPGSPGPPGSFDFLLLVLADIRNDIAELQEKVFGHRTHSSAEDFP. The peptide sequence for this immunogen was taken from within the described region.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Scientific Data Images for CCBE1 Antibody

Western Blot: CCBE1 Antibody [NBP1-79501]

Western Blot: CCBE1 Antibody [NBP1-79501]

Western Blot: CCBE1 Antibody [NBP1-79501] - Titration: 1.0 ug/ml Positive Control: Mouse Kidney.
CCBE1 Antibody

Western Blot: CCBE1 Antibody [NBP1-79501] -

Western Blot: CCBE1 Antibody [NBP1-79501] - Hsa_circ_0076931/miR-6760-3p/CCBE1 axis is predicted to play important roles in the progression of glioma(A) circRNA/miRNA/mRNA ceRNA network analysis. (B) The level of CCBE1 in H4 & U118-MG cells was detected by qRT-PCR after transfection with hsa_circ_0076931. (C) The level of CCBE1 in H4 & U118-MG cells was detected by Western blot after transfection with hsa_circ_0076931. (D) Quantification of CCBE1 protein is shown. (E) The level of miR-6067-3p in H4 & U118-MG cells was detected by qRT-PCR after transfection with hsa_circ_0076931. (F) Schematic of the binding site between hsa_circ_0076931 & miR-6067-3p, & the luciferase activity of WT hsa_circ_0076931 3′ UTR or mutant hsa_circ_0076931 3′ UTR after transfection with miR-6067-3p mimics in 293T cells. (G) Schematic of the binding site between miR-6067-3p & CCBE1. (H) AGO2-RIP assay was used to analyze the expression of hsa_circ_0076931, miR-6760-3p, & CCBE1 in U118-MG cells. (I) After interference with hsa_circ_0076931, the expression of CCBE1 was tested by AGO2-RIP assay in U118-MG cells; *P<0.05, **P<0.01, ***P<0.001. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/34931668), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for CCBE1 Antibody

Application
Recommended Usage

Western Blot

1.0 ug/ml

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 2% Sucrose

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CCBE1

CCBE1 is required for lymphangioblast budding and angiogenic sprouting from venous endothelium during embryogenesis

Long Name

Collagen And Calcium Binding EGF Domains 1

Alternate Names

HKLLS1, KIAA1983

Gene Symbol

CCBE1

Additional CCBE1 Products

Product Documents for CCBE1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CCBE1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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