CCDC109A Antibody (CL3576)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-59029
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human
Predicted:
Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
Applications
Immunocytochemistry/ Immunofluorescence, Knockdown Validated, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # CL3576
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against a recombinant protein corresponding to the following amino acid sequence:TRQEYVYPEARDRQYLLFFHKGAKKSRFDLEKYNQLKDAIAQAEMDLKRLRDPLQVHLPLRQIGE
Epitope
RDRQYLLFFH
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for CCDC109A Antibody (CL3576)
![Western Blot: CCDC109A Antibody (CL3576) [NBP2-59029]](https://resources.bio-techne.com/images/products/CCDC109A-Antibody-CL3576-Western-Blot-NBP2-59029-img0002.jpg "Western Blot: CCDC109A Antibody (CL3576) [NBP2-59029]")
Western Blot: CCDC109A Antibody (CL3576) [NBP2-59029]
Western Blot: CCDC109A Antibody (CL3576) [NBP2-59029] - Analysis of extracts from A-549 cells, transfected with either control siRNA (siRNA ctrl) or target specific siRNA probes (siRNA#1, siRNA#2), using Anti-MCU monoclonal antibody. Downregulation of antibody signal, relative control lane (% intensity), confi![Immunocytochemistry/ Immunofluorescence: CCDC109A Antibody (CL3576) [NBP2-59029]](https://resources.bio-techne.com/images/products/CCDC109A-Antibody-CL3576-Immunocytochemistry-Immunofluorescence-NBP2-59029-img0004.jpg "Immunocytochemistry/ Immunofluorescence: CCDC109A Antibody (CL3576) [NBP2-59029]")
Immunocytochemistry/ Immunofluorescence: CCDC109A Antibody (CL3576) [NBP2-59029]
Immunocytochemistry/Immunofluorescence: CCDC109A Antibody (CL3576) [NBP2-59029] - Staining of A549 cells using the Anti-MCU monoclonal antibody, showing specific staining in the mitochondria in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available). Antibody staining is shown in green.Applications for CCDC109A Antibody (CL3576)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
2-10 ug/ml
Western Blot
1 ug/ml
Application Notes
ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: CCDC109A
Long Name
Calcium uniporter protein, mitochondrial
Alternate Names
C10orf42, HsMCU, MCU
Gene Symbol
MCU
Additional CCDC109A Products
Product Documents for CCDC109A Antibody (CL3576)
Product Specific Notices for CCDC109A Antibody (CL3576)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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