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CCDC124 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-48858

Novus Biologicals, part of Bio-Techne
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NBP2-48858
NBP2-48858-25ul

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: LEDAYWKDDDKHVMRKEQRKEEKEKRRLDQLERKKETQRLLEEEDSKLKGGKAPRVATSSKVTRAQIEDTLRRDHQLRE

Reactivity Notes

Mouse (84%), Rat (82%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CCDC124 Antibody

Immunocytochemistry/ Immunofluorescence: CCDC124 Antibody [NBP2-48858]

Immunocytochemistry/ Immunofluorescence: CCDC124 Antibody [NBP2-48858]

Immunocytochemistry/Immunofluorescence: CCDC124 Antibody [NBP2-48858] - Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & cytosol.
Immunohistochemistry-Paraffin: CCDC124 Antibody [NBP2-48858]

Immunohistochemistry-Paraffin: CCDC124 Antibody [NBP2-48858]

Immunohistochemistry-Paraffin: CCDC124 Antibody [NBP2-48858] - Staining of human hippocampus shows strong cytoplasmic positivity in neuronal cells.
CCDC124 Antibody

Western Blot: CCDC124 Antibody [NBP2-48858] -

Western Blot: CCDC124 Antibody [NBP2-48858] - The expression of IL‐25 affects the cisplatin resistance of lung cancer cells. A, The expression of IL‐25 in A549 & A549/CDDP cells. B, IL‐25 overexpressed in A549 cells. C, silence of IL‐25 expression by siRNA in A549/CDDP cells. D, IL‐25 overexpression desensitizes A549 cells to cisplatin treatment. E, Silence of IL‐25 expression sensitizes A549/CDDP cells to cisplatin treatment. *P < 0.01 vs indicated controls, all figures represent the average of three sets of independent experiments Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31044552), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for CCDC124 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:2500 - 1:5000

Immunohistochemistry-Paraffin

1:2500 - 1:5000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100. Use in Western blot reported in scientific literature (PMID: 31044552).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CCDC124

CCDC124 is a coiled-coil domain containing protein of unknown function.

Alternate Names

coiled-coil domain containing 124, coiled-coil domain-containing protein 124

Gene Symbol

CCDC124

Additional CCDC124 Products

Product Documents for CCDC124 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CCDC124 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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