CCDC137 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-12572
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Produced in rabbits immunized with E. coli-derived Human CCDC137 fragment
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Protein A & Antigen Affinity purified
Protein A & Antigen Affinity purified
Scientific Data Images for CCDC137 Antibody
Western Blot: CCDC137 Antibody [NBP3-12572]
Western Blot: CCDC137 Antibody [NBP3-12572] - Lane A: A549 Whole Cell LysateLane B: H1299 Whole Cell LysateLane C: HCT116 Whole Cell LysateLane D: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:33 kDaObserved band size:38 kDaImmunocytochemistry/ Immunofluorescence: CCDC137 Antibody [NBP3-12572]
Immunocytochemistry/Immunofluorescence: CCDC137 Antibody [NBP3-12572] - Staining of CCDC137 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human CCDC137 polyclonal antibody (dilution ratio 1:200) at 4? overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Nucleus.Immunohistochemistry-Paraffin: CCDC137 Antibody [NBP3-12572]
Immunohistochemistry-Paraffin: CCDC137 Antibody [NBP3-12572] - Staining of human CCDC137 in human skeletal muscle with rabbit polyclonal antibody at 1:100 dilution.Applications for CCDC137 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:500
Immunohistochemistry-Paraffin
1:50-1:200
Immunoprecipitation
0.5-1uL/mg of uLysate
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
PBS
Preservative
0.03% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: CCDC137
Alternate Names
coiled-coil domain containing 137, coiled-coil domain-containing protein 137, MGC16597
Gene Symbol
CCDC137
Additional CCDC137 Products
Product Specific Notices for CCDC137 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...