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CCDC137 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-12572

Novus Biologicals, part of Bio-Techne
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NBP3-12572-50ul
NBP3-12572-100ul

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Produced in rabbits immunized with E. coli-derived Human CCDC137 fragment

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Protein A & Antigen Affinity purified

Scientific Data Images for CCDC137 Antibody

Western Blot: CCDC137 Antibody [NBP3-12572]

Western Blot: CCDC137 Antibody [NBP3-12572]

Western Blot: CCDC137 Antibody [NBP3-12572] - Lane A: A549 Whole Cell LysateLane B: H1299 Whole Cell LysateLane C: HCT116 Whole Cell LysateLane D: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:33 kDaObserved band size:38 kDa
Immunocytochemistry/ Immunofluorescence: CCDC137 Antibody [NBP3-12572]

Immunocytochemistry/ Immunofluorescence: CCDC137 Antibody [NBP3-12572]

Immunocytochemistry/Immunofluorescence: CCDC137 Antibody [NBP3-12572] - Staining of CCDC137 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human CCDC137 polyclonal antibody (dilution ratio 1:200) at 4? overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Nucleus.
Immunohistochemistry-Paraffin: CCDC137 Antibody [NBP3-12572]

Immunohistochemistry-Paraffin: CCDC137 Antibody [NBP3-12572]

Immunohistochemistry-Paraffin: CCDC137 Antibody [NBP3-12572] - Staining of human CCDC137 in human skeletal muscle with rabbit polyclonal antibody at 1:100 dilution.

Applications for CCDC137 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:500

Immunohistochemistry-Paraffin

1:50-1:200

Immunoprecipitation

0.5-1uL/mg of uLysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

PBS

Preservative

0.03% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CCDC137

Alternate Names

coiled-coil domain containing 137, coiled-coil domain-containing protein 137, MGC16597

Gene Symbol

CCDC137

Additional CCDC137 Products

Product Documents for CCDC137 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CCDC137 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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