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CCDC186 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-90440

Novus Biologicals, part of Bio-Techne
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NBP1-90440
NBP1-90440-25ul

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse, Rat

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LKRTFKEGMDELRTLRTKVKCLEDERLRTEDELSKYKEIINRQKAEIQNLLDKVKTADQLQEQLQRGKQEIENLKEEVESLNSLINDLQKDIEGSRKRESELLLFTERLTSKNAQLQSESNSLQSQFDKVSC

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CCDC186 Antibody

Western Blot: CCDC186 Antibody [NBP1-90440]

Western Blot: CCDC186 Antibody [NBP1-90440]

Western Blot: CCDC186 Antibody [NBP1-90440] - Analysis in Rh30 cells transfected with control siRNA, target specific siRNA probe #1 and #2,. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Immunocytochemistry/ Immunofluorescence: CCDC186 Antibody [NBP1-90440]

Immunocytochemistry/ Immunofluorescence: CCDC186 Antibody [NBP1-90440]

Immunocytochemistry/Immunofluorescence: CCDC186 Antibody [NBP1-90440] - Staining of human cell line A-431 shows localization to the Golgi apparatus. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: CCDC186 Antibody [NBP1-90440]

Immunohistochemistry-Paraffin: CCDC186 Antibody [NBP1-90440]

Immunohistochemistry-Paraffin: CCDC186 Antibody [NBP1-90440] - Staining of human duodenum shows strong cytoplasmic positivity in glandular cells.

Applications for CCDC186 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CCDC186

Alternate Names

C10orf118, chromosome 10 open reading frame 118, FLJ35301, MGC129699

Gene Symbol

CCDC186

Additional CCDC186 Products

Product Documents for CCDC186 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CCDC186 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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