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CCDC5 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-88159

Novus Biologicals, part of Bio-Techne
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NBP1-88159
NBP1-88159-25ul

Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: QEERETQVAAWLKKIFGDHPIPQYEVNPRTTEILHHLSERNRVRDRDVYLVIEDLKQKASEYESEAKYLQDLLMESVNFSPANLSSTGSRYLNALVD

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (82%), Rat (85%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CCDC5 Antibody

Western Blot: CCDC5 Antibody [NBP1-88159]

Western Blot: CCDC5 Antibody [NBP1-88159]

Western Blot: CCDC5 Antibody [NBP1-88159] - Analysis using Anti-HAUS1 antibody NBP1-88159 (A) shows similar pattern to independent antibody NBP1-88158 (B).
Immunocytochemistry/ Immunofluorescence: CCDC5 Antibody [NBP1-88159]

Immunocytochemistry/ Immunofluorescence: CCDC5 Antibody [NBP1-88159]

Immunocytochemistry/Immunofluorescence: CCDC5 Antibody [NBP1-88159] - Staining of human cell line U-2 OS shows localization to cytosol & centrosome. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: CCDC5 Antibody [NBP1-88159]

Immunohistochemistry-Paraffin: CCDC5 Antibody [NBP1-88159]

Immunohistochemistry-Paraffin: CCDC5 Antibody [NBP1-88159] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.

Applications for CCDC5 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CCDC5

CCDC5 belongs to the HAUS1 family. More specifically, CCDC5 is a component of the HAUS augmen complex. Originally founded in Drosophila, the complex plays an important role in spindle assembly, maintenance of centrosome integrity and completion of cytokinesis.

Alternate Names

CCDC5Enhancer of invasion-cluster, coiled-coil domain containing 5 (spindle associated), FLJ40084, HAUS augmin-like complex subunit 1, HAUS augmin-like complex, subunit 1, HEI-CCoiled-coil domain-containing protein 5, HEICFLJ21094, HsT1461

Gene Symbol

HAUS1

Additional CCDC5 Products

Product Documents for CCDC5 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CCDC5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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