CCDC8 Antibody

Western Blot: CCDC8 Antibody [NBP1-50021]

Western Blot: CCDC8 Antibody [NBP1-50021] - Detection of Human CCDC8 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from 293T (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and K562 (K; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-CCDC8 antibody used for WB at 0.04 mcg/ml (A) and 0.1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. CCDC8 was also immunoprecipitated by rabbit anti-CCDC8 antibody which recognizes a downstream epitope. For blotting immunoprecipitated CCDC8, the ReliaBLOT (R) Reagents and Procedures were used. Detection: Chemiluminescence with exposure times of 10 seconds (A and B).