CCDC82 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-83583
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Summary for CCDC82 Antibody
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: DSEEFDSDEELDSDESFENDEELDSNKGPDCNKTPGSERELNLSKIQSEGNDSKCLINSGNGSTYEEETNKIKHRNIDLQDQEKHLSQEDNDLNKQT
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for CCDC82 Antibody
Western Blot: CCDC82 Antibody [NBP1-83583]
Western Blot: CCDC82 Antibody [NBP1-83583] - Analysis in control (vector only transfected HEK293T lysate) and CCDC82 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).Immunocytochemistry/ Immunofluorescence: CCDC82 Antibody [NBP1-83583]
Immunocytochemistry/Immunofluorescence: CCDC82 Antibody [NBP1-83583] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus, nucleoli & cytosol.Immunohistochemistry-Paraffin: CCDC82 Antibody [NBP1-83583]
Immunohistochemistry-Paraffin: CCDC82 Antibody [NBP1-83583] - Staining of human smooth muscle shows strong cytoplasmic positivity in smooth muscle cells.Applications for CCDC82 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:200 - 1:500
Immunohistochemistry-Paraffin
1:200 - 1:500
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: CCDC82
Alternate Names
coiled-coil domain containing 82, coiled-coil domain-containing protein 82, FLJ23518, HSPC048
Gene Symbol
CCDC82
Additional CCDC82 Products
Product Documents for CCDC82 Antibody
Product Specific Notices for CCDC82 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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