CCL7/MCP-3/MARC Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-82367

Novus Biologicals, part of Bio-Techne
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NBP1-82367
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.5 mg/ml

View all CCL7/MCP-3/MARC Antibodies »

Product Specifications

Immunogen

Synthetic peptide towards MCP3. Peptide sequence TSTTCCYRFINKKIPKQRLESYRRTTSSHCPREAVIFKTKLDKEICADPT. The peptide sequence for this immunogen was taken from within the described region.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Scientific Data Images for CCL7/MCP-3/MARC Antibody

Western Blot: CCL7/MCP-3/MARC Antibody [NBP1-82367]

Western Blot: CCL7/MCP-3/MARC Antibody [NBP1-82367]

Western Blot: CCL7/MCP-3/MARC Antibody [NBP1-82367] - Reccomended Titration: 0.2 - 1 ug/ml Positive Control: Jurkat cell lysate
Immunohistochemistry-Frozen: CCL7/MCP-3/MARC Antibody [NBP1-82367]

Immunohistochemistry-Frozen: CCL7/MCP-3/MARC Antibody [NBP1-82367]

Immunohistochemistry-Frozen: CCL7/MCP-3/MARC Antibody [NBP1-82367] - CCL7 (green) stained frozen human skin section, counterstained with DAPI (40x). Sections fixed with 4% PFA PBS, permeabilized with 0.5% Triton TBS Ca/Azide, and blocked with 5% BSA TBS Ca/Azide at 37C for 1 hour. Antibody diluted 1:50 in block, and incubated for 1 hour at 37C. Sections were washed and stained with secondary antibody, and counterstained with DAPI. Submitted by a verified customer review.
Western Blot: CCL7/MCP-3/MARC Antibody [NBP1-82367]

Western Blot: CCL7/MCP-3/MARC Antibody [NBP1-82367]

Western Blot: CCL7/MCP-3/MARC Antibody [NBP1-82367] - Fetal Small Intestine.
View 1 more images

Applications for CCL7/MCP-3/MARC Antibody

Application
Recommended Usage

Immunohistochemistry-Frozen

1:50

Western Blot

1.0 ug/ml
Application Notes
Validated for use in IHC-F reported by a verified customer review.

Reviewed Applications

Read 1 review rated 5 using NBP1-82367 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 2% Sucrose

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CCL7/MCP-3/MARC

MCP3 encodes monocyte chemotactic protein 3, a secreted chemokine which attracts macrophages during inflammation and metastasis. It is a member of the C-C subfamily of chemokines which are characterized by having two adjacent cysteine residues. The protein is an in vivo substrate of matrix metalloproteinase 2, an enzyme which degrades components of the extracellular matrix. This gene is part of a cluster of C-C chemokine family members on chromosome 17q.

Alternate Names

MARC, MCP-3

Gene Symbol

CCL7

UniProt

NP_006264 (Human)

Additional CCL7/MCP-3/MARC Products

Product Documents for CCL7/MCP-3/MARC Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CCL7/MCP-3/MARC Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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