CCL8/MCP-2 Antibody (CCL8/3683)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13770

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2c Kappa Clone # CCL8/3683

Concentration

0.2 mg/ml

View all CCL8/MCP-2 Antibodies »

Product Summary for CCL8/MCP-2 Antibody (CCL8/3683)

Immunogen

Human recombinant CCL8/MCP-2 protein fragment (around aa1-99) (exact sequence is proprietary) (Uniprot: P80075)

Localization

Secreted

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2c Kappa

Theoretical MW

11 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14173)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for CCL8/MCP-2 Antibody (CCL8/3683)

Immunohistochemistry-Paraffin: CCL8/MCP-2 Antibody (CCL8/3683) [NBP3-13770]

Immunohistochemistry-Paraffin: CCL8/MCP-2 Antibody (CCL8/3683) [NBP3-13770]

Immunohistochemistry-Paraffin: CCL8/MCP-2 Antibody (CCL8/3683) [NBP3-13770] - Formalin-fixed, paraffin-embedded human adrenal gland stained with CCL8/MCP-2 antibody (CCL8/3683). HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.

Applications for CCL8/MCP-2 Antibody (CCL8/3683)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: CCL8/MCP-2

MCP2 and MCP3 are two recently identified monocyte chemotactic proteins produced by human MG63 osteosarcoma cells. Both MCP2 and MCP3 are members of the C-C family of chemokines and share 62% and 71% amino acid sequence identity, respectively, with MCP1. MCP3 also shares 58% amino acid identity with MCP2. Similarly to other C-C chemokines, all three MCP proteins are monocyte chemoattractants. In addition, the three MCPs can chemoattract activated NK cells as well as CD4+ and CD8+ T lymphocytes. All three cytokines have also been shown to attract eosinophils and induce histamine secretion from basophils.

Alternate Names

MCP-2, MCP2

Gene Symbol

CCL8

UniProt

P80075

Additional CCL8/MCP-2 Products

Product Documents for CCL8/MCP-2 Antibody (CCL8/3683)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for CCL8/MCP-2 Antibody (CCL8/3683)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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