Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all Cyclin K/CCNK Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 530 and 580 of human cyclin K using the numbering given in entry NP_001092872.1 (GeneID 8812).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CCNK Antibody

Western Blot: CCNK Antibody [NBP1-06519]

Western Blot: CCNK Antibody [NBP1-06519]

Western Blot: CCNK Antibody [NBP1-06519] - Whole cell lysate (50 ug) from NIH 3T3, CH27, TCMK-1, BW5147.3, and CT26 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Cyclin Kantibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunohistochemistry-Paraffin: CCNK Antibody [NBP1-06519]

Immunohistochemistry-Paraffin: CCNK Antibody [NBP1-06519]

Immunohistochemistry-Paraffin: CCNK Antibody [NBP1-06519] - Affinity purified rabbit anti-Cyclin K used at a dilution of 1:5,000 (0.2 ug/ml). Detection: DAB
Western Blot: CCNK Antibody [NBP1-06519]

Western Blot: CCNK Antibody [NBP1-06519]

Western Blot: CCNK Antibody [NBP1-06519] - Whole cell lysate (50 ug) from HEK293T, MCF-7, Jurkat, HeLa, and Hep-G2 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Cyclin K antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.
View 1 more images

Applications for CCNK Antibody

Application
Recommended Usage

Immunohistochemistry

1:1000 - 1:5000

Immunohistochemistry-Paraffin

1:1000 - 1:5000

Immunoprecipitation

2 -10 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Cyclin K/CCNK

The protein encoded by this gene is a member of the transcription cyclin family. These cyclins may regulate transcription through their association with and activation of cyclin-dependent kinases (CDK) that phosphorylate the C-terminal domain (CTD) of the large subunit of RNA polymerase II. This gene product may play a dual role in regulating CDK and RNA polymerase II activities. [provided by RefSeq]

Long Name

Cyclin K

Alternate Names

CCNK, CPR4, IDDHDF

Entrez Gene IDs

8812 (Human)

Gene Symbol

CCNK

UniProt

O75909 (Human)

Additional Cyclin K/CCNK Products

Product Documents for CCNK Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for CCNK Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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