CCT3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-51949

Novus Biologicals, part of Bio-Techne
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NBP1-51949
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Key Product Details

Species Reactivity

Validated:

Mouse, Rat

Predicted:

Bovine (100%), Canine (100%), Human (100%), Porcine (100%). Backed by our 100% Guarantee.

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all CCT3 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-GHKKKGDDQSRQGG corresponding to C-Terminus (near) according to NP_001008883.1, NP_001008800.1.

Epitope

C-GHKKKGDDQSRQGG

Specificity

This antibody is expected to recognize all reported isoforms (NP_005989.3; NP_001008883.1; NP_001008800.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for CCT3 Antibody

Western Blot: CCT3 Antibody [NBP1-51949]

Western Blot: CCT3 Antibody [NBP1-51949]

Western Blot: CCT3 Antibody [NBP1-51949] - Analysis of CCT3 in Mouse Testis lysate (35ug protein in RIPA buffer) using this antibody at 0.01 ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for CCT3 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:32000

Western Blot

0.01 - 0.03 ug/ml
Application Notes
WB: Approx. 65 kDa band observed in mouse and rat testis lysates (calculated MW of 60.5 kDa band according to human NP_005989.3 and 60.6 kDa band according to mouse NP_033966.1 and rat NP_954522.1).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CCT3

This gene encodes a molecular chaperone that is member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]

Alternate Names

Cctg, chaperonin containing TCP1, subunit 3 (gamma), hTRiC5, TCP1 (t-complex-1) ring complex, polypeptide 5, TRIC5gamma subunit

Entrez Gene IDs

7203 (Human); 12462 (Mouse); 295230 (Rat)

Gene Symbol

CCT3

UniProt

NP_005989.3 (Human)

Additional CCT3 Products

Product Documents for CCT3 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for CCT3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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