CCT3 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-79041
Key Product Details
Validated by
Independent Antibodies, Biological Validation
Species Reactivity
Validated:
Human, Mouse
Predicted:
Bovine (100%), Orangutan (100%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
0.2 mg/ml
Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 495 and 545 of human Chaperonin containing TCP1, Subunit 3 using the numbering given in entry NP_005989.3 (GeneID 7203).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for CCT3 Antibody
![Western Blot: CCT3 Antibody [NBP1-79041]](https://resources.bio-techne.com/images/products/CCT3-Antibody-Western-Blot-NBP1-79041-img0001.jpg "Western Blot: CCT3 Antibody [NBP1-79041]")
Western Blot: CCT3 Antibody [NBP1-79041]
Western Blot: CCT3 Antibody [NBP1-79041] - Whole cell lysate from HeLa (15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), Jurkat (J; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-CCT3 antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 6 mcg/mg lysate. CCT3 was also immunoprecipitated by rabbit anti-CCT3 antibody NBP1-79040 which recognizes an upstream epitope.![Immunohistochemistry-Paraffin: CCT3 Antibody [NBP1-79041]](https://resources.bio-techne.com/images/products/CCT3-Antibody-Immunohistochemistry-NBP1-79041-img0009.jpg "Immunohistochemistry-Paraffin: CCT3 Antibody [NBP1-79041]")
Immunohistochemistry-Paraffin: CCT3 Antibody [NBP1-79041]
Immunohistochemistry-Paraffin: CCT3 Antibody [NBP1-79041] - Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti- CCT3 used at a dilution of 1:200 (1ug/ml). Detection: DAB. Counterstain: hematoxylin (blue).![Immunohistochemistry-Paraffin: CCT3 Antibody [NBP1-79041]](https://resources.bio-techne.com/images/products/CCT3-Antibody-Immunohistochemistry-NBP1-79041-img0008.jpg "Immunohistochemistry-Paraffin: CCT3 Antibody [NBP1-79041]")
Immunohistochemistry-Paraffin: CCT3 Antibody [NBP1-79041]
Immunohistochemistry-Paraffin: CCT3 Antibody [NBP1-79041] - Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti- CCT3 used at a dilution of 1:200 (1ug/ml). Detection: DAB. Counterstain: hematoxylin (blue).Applications for CCT3 Antibody
Application
Recommended Usage
Immunohistochemistry
1:100 - 1:500
Immunohistochemistry-Paraffin
1:100- 1:500
Immunoprecipitation
2-10ug/mg
Western Blot
1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: CCT3
Alternate Names
Cctg, chaperonin containing TCP1, subunit 3 (gamma), hTRiC5, TCP1 (t-complex-1) ring complex, polypeptide 5, TRIC5gamma subunit
Gene Symbol
CCT3
UniProt
Additional CCT3 Products
Product Documents for CCT3 Antibody
Product Specific Notices for CCT3 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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