CCT3 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-99266
![Western Blot: CCT3 Antibody [NBP2-99266]](https://resources.bio-techne.com/images/products/CCT3-Antibody-Western-Blot-NBP2-99266-img0002.jpg "Western Blot: CCT3 Antibody [NBP2-99266]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Cynomolgus Monkey (100%), Rat (100%). Backed by our 100% Guarantee.
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for CCT3 Antibody
Immunogen
Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human CCT3.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for CCT3 Antibody
Western Blot: CCT3 Antibody [NBP2-99266]
Western Blot: CCT3 Antibody [NBP2-99266] - Anti-CCT3 rabbit polyclonal antibody at 1:500 dilution. Lane A: K562 Whole Cell Lysate Lane B: HL-60 Whole Cell Lysate Lane C: HeLa Whole Cell Lysate Lane D: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 61 kDa. Observed band size: 61 kDa![Immunocytochemistry/ Immunofluorescence: CCT3 Antibody [NBP2-99266]](https://resources.bio-techne.com/images/products/CCT3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-99266-img0001.jpg "Immunocytochemistry/ Immunofluorescence: CCT3 Antibody [NBP2-99266]")
Immunocytochemistry/ Immunofluorescence: CCT3 Antibody [NBP2-99266]
Immunocytochemistry/Immunofluorescence: CCT3 Antibody [NBP2-99266] - Immunofluorescence staining of CCT3 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human CCT3 polyclonal antibody (dilution ratio 1:500) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Cytoplasm.Applications for CCT3 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:500
Western Blot
1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
PBS
Preservative
0.03% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: CCT3
Alternate Names
Cctg, chaperonin containing TCP1, subunit 3 (gamma), hTRiC5, TCP1 (t-complex-1) ring complex, polypeptide 5, TRIC5gamma subunit
Gene Symbol
CCT3
Additional CCT3 Products
Product Specific Notices for CCT3 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...