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CCT5 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-83042

Novus Biologicals, part of Bio-Techne
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NBP1-83042-25ul
NBP1-83042

Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: ALQKYEKEKFEEMIQQIKETGANLAICQWGFDDEANHLLLQNNLPAVRWVGGPEIELIAIATGGRIVPRFSELTAEKLGFAGLVQEISFGTTKDKMLVIEQCKNSRAVTIFIRGGNKMIIEEAKRSLHDALCVIRNLIRDNRVVYGGGA

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CCT5 Antibody

Immunohistochemistry-Paraffin: CCT5 Antibody [NBP1-83042]

Immunohistochemistry-Paraffin: CCT5 Antibody [NBP1-83042]

Immunohistochemistry-Paraffin: CCT5 Antibody [NBP1-83042] - Staining in human testis and liver tissues using anti-CCT5 antibody. Corresponding CCT5 RNA-seq data are presented for the same tissues.
Western Blot: CCT5 Antibody [NBP1-83042]

Western Blot: CCT5 Antibody [NBP1-83042]

Western Blot: CCT5 Antibody [NBP1-83042] - Analysis using Anti-CCT5 antibody NBP1-83042 (A) shows similar pattern to independent antibody NBP1-83043 (B).
Immunohistochemistry-Paraffin: CCT5 Antibody [NBP1-83042]

Immunohistochemistry-Paraffin: CCT5 Antibody [NBP1-83042]

Immunohistochemistry-Paraffin: CCT5 Antibody [NBP1-83042] - Staining of human cerebral cortex, colon, liver and testis using Anti-CCT5 antibody NBP1-83042 (A) shows similar protein distribution across tissues to independent antibody NBP1-83043 (B).

Applications for CCT5 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CCT5

CCT5 encodes a molecular chaperone that is member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized.

Alternate Names

chaperonin containing TCP1, subunit 5 (epsilon), epsilon subunit

Gene Symbol

CCT5

Additional CCT5 Products

Product Documents for CCT5 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CCT5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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