CD-M6PR Antibody (PSH01-06)

Western Blot: CD-M6PR Antibody (PSH01-06) [NBP3-32555]

Western Blot: CD-M6PR Antibody (PSH01-06) [NBP3-32555] - Western blot analysis of CD-M6PR on different lysates with Rabbit anti-CD-M6PR antibody (NBP3-32555) at 1/1,000 dilution.

Lane 1: A549 cell lysate (20 ug/Lane)
Lane 2: PC-12 cell lysate (20 ug/Lane)
Lane 3: Mouse kidney tissue lysate (40 ug/Lane)
Lane 4: Rat kidney tissue lysate (40 ug/Lane)

Predicted band size: 31 kDa
Observed band size: 37/46 kDa

Exposure time: 3 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32555) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: CD-M6PR Antibody (PSH01-06) [NBP3-32555]

Immunohistochemistry: CD-M6PR Antibody (PSH01-06) [NBP3-32555] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-CD-M6PR antibody (NBP3-32555) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32555) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.