CD117/c-kit Antibody (YB5.B8) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-43358
Clone YB5.B8 was used by HLDA to establish CD designation.
![Flow Cytometry: CD117/c-kit Antibody (YB5.B8) - BSA Free [NBP1-43358]](https://resources.bio-techne.com/images/products/CD117-c-kit-Antibody-YB5-B8-Flow-Cytometry-NBP1-43358-img0004.jpg "Flow Cytometry: CD117/c-kit Antibody (YB5.B8) - BSA Free [NBP1-43358]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunoprecipitation
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # YB5.B8
Format
BSA Free
Concentration
5 mg/ml
Product Specifications
Immunogen
Human CD117
Reactivity Notes
This antibody is reactive to Human.
Marker
Hematopoietic Stem Cell Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images for CD117/c-kit Antibody (YB5.B8) - BSA Free
Flow Cytometry: CD117/c-kit Antibody (YB5.B8) - BSA Free [NBP1-43358]
Flow Cytometry: CD117/c-kit Antibody (YB5.B8) [NBP1-43358] - Analysis using the DyLight 405 conjugate of NBP1-43358. Staining of CD117 in human PBMCs using anti-CD117 antibody. Image from verified customer review.![Flow Cytometry: CD117/c-kit Antibody (YB5.B8) - BSA Free [NBP1-43358]](https://resources.bio-techne.com/images/products/CD117-c-kit-Antibody-YB5-B8-Flow-Cytometry-NBP1-43358-img0003.jpg "Flow Cytometry: CD117/c-kit Antibody (YB5.B8) - BSA Free [NBP1-43358]")
Flow Cytometry: CD117/c-kit Antibody (YB5.B8) - BSA Free [NBP1-43358]
Flow Cytometry: CD117/c-kit Antibody (YB5.B8) [NBP1-43358] - Analysis using the Biotin conjugate of NBP1-43358. Staining of TF-1 cell line with 0.5 ug of Mouse IgG1 kappa Isotype Control Biotin (open histogram) or 0.5 ug of Anti-Human CD117 (c-Kit) Biotin (filled histogram) followed by Streptavidin PE.Applications for CD117/c-kit Antibody (YB5.B8) - BSA Free
Application
Recommended Usage
Flow Cytometry
1:10-1:1000
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Frozen
1:10-1:500
Immunoprecipitation
1:10-1:500
Application Notes
The YB5.B8 antibody has been tested by flow cytometric analysis of the TF-1 cell line, a human erythroleukemia cell line. This can be used at less than or equal to 1 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
PBS (pH 7.2)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: CD117/c-kit
Long Name
Stem Cell Factor Receptor
Alternate Names
c-kit, CD117, ckit, KIT, PBT, SCF R, SCFR
Gene Symbol
KIT
Additional CD117/c-kit Products
Product Documents for CD117/c-kit Antibody (YB5.B8) - BSA Free
Product Specific Notices for CD117/c-kit Antibody (YB5.B8) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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