CD11a/CD18 Antibody (hu1124 (Efalizumab)) - Chimeric - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-52686

Recombinant Monoclonal Antibody.
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Block/Neutralize, CyTOF-ready, Flow Cytometry, Immunohistochemistry

Cited:

Block/Neutralize

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # hu1124 (Efalizumab)

Format

Azide and BSA Free

Concentration

1 mg/ml

View all CD11a/CD18 Antibodies »

Product Specifications

Immunogen

Efalizumab binds to CD11a, a subunit of leukocyte function antigen-1 (LFA-1), which is expressed on all leukocytes.

Specificity

Binds to human LFA-1.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Scientific Data Images

Immunohistochemistry: CD11a/CD18 Antibody (hu1124 (Efalizumab)) - Chimeric - Azide and BSA Free [NBP2-52686]

Immunohistochemistry: CD11a/CD18 Antibody (hu1124 (Efalizumab)) - Chimeric - Azide and BSA Free [NBP2-52686]

Immunohistochemistry: CD11a/CD18 Antibody (hu1124 (Efalizumab)) - Chimeric [NBP2-52686] - Anti-CD11a staining of formaldehyde fixed paraffin embedded rat spleen tissue, at 40x magnification. The human IgG1-chimeric version of hu1124 (NBP2-75912) was used to stain samples at a concentration of 3 ug/ml.
Flow Cytometry: CD11a/CD18 Antibody (hu1124 (Efalizumab)) - Chimeric - Azide and BSA Free [NBP2-52686]

Flow Cytometry: CD11a/CD18 Antibody (hu1124 (Efalizumab)) - Chimeric - Azide and BSA Free [NBP2-52686]

Flow Cytometry: CD11a/CD18 Antibody (hu1124 (Efalizumab)) - Chimeric [NBP2-52686] - Flow-cytometry using the anti-CD11a research biosimilar antibody Efalizumab. Human lymphocytes were stained with an isotype control (NBP2-52638, panel A) or the rabbit-chimeric version of Efalizumab (NBP2-52686, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.

Applications

Application
Recommended Usage

Flow Cytometry

1:10 - 1:1000
Application Notes
This antibody is Cytof ready. This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques. Efalizumab binds to the LFA-1 alpha I domain blocking the ICAM-1 binding site via steric hindrance.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.

Background: CD11a/CD18

The CD11/18 complex of glycoproteins are dimers comprised of a common beta chain and individual alpha chains (CD11a/18 or CD11b/18 and CD11c/18). CD11a/18 is a receptor for C3bi, a complement component, CD11b/18 is Lymphocyte Function Activator, and CDD11c/18 is a lectin-like glycoprotein thought to be important for mediation of cells to other surfaces.

Alternate Names

CD11 antigen-like family member A, CD11a antigen, CD11Aantigen CD11A (p180), lymphocyte function-associated antigen 1, alphapolypeptide, integrin alpha-L, integrin gene promoter, integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen1; alpha polypeptide), Leukocyte adhesion glycoprotein LFA-1 alpha chain, Leukocyte function-associated molecule 1 alpha chain, LFA-1, LFA-1 alpha, LFA1A, LFA-1A, lymphocyte function-associated antigen 1, biosimilar antibody, biosimilars

Gene Symbol

ITGAL

Additional CD11a/CD18 Products

Product Documents

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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