CD133 Antibody (3Y4A5)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15310

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15310-100ul
NBP3-15310-20ul
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Knockout Validated, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 3Y4A5

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CD133 Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 250-380 of human CD133 (O43490). IPVLDEIKSMATAIKETKEALENMNSTLKSLHQQSTQLSSSLTSVKTSLRSSLNDPLCLVHPSSETCNSIRLSLSQLNSNPELRQLPPVDAELDNVNNVLRTDLDGLVQQGYQSLNDIPDRVQRQTTTVVA

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CD133 Antibody (3Y4A5)

Western Blot: CD133 Antibody (3Y4A5) [NBP3-15310]

Western Blot: CD133 Antibody (3Y4A5) [NBP3-15310]

Western Blot: CD133 Antibody (3Y4A5) [NBP3-15310] - Western blot analysis of extracts of HT-29 cells, using CD133 antibody (NBP3-15310) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunohistochemistry-Paraffin: CD133 Antibody (3Y4A5) [NBP3-15310]

Immunohistochemistry-Paraffin: CD133 Antibody (3Y4A5) [NBP3-15310]

Immunohistochemistry-Paraffin: CD133 Antibody (3Y4A5) [NBP3-15310] - Immunohistochemistry of paraffin-embedded human colon carcinoma using CD133 Rabbit mAb (NBP3-15310) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Knockout Validated: CD133 Antibody (3Y4A5) [NBP3-15310]

Knockout Validated: CD133 Antibody (3Y4A5) [NBP3-15310]

Knockout Validated: CD133 Antibody (3Y4A5) [NBP3-15310] - Western blot analysis of extracts from normal (control) and CD133 knockout (KO) HCT116 cells, using CD133 antibody (NBP3-15310) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Applications for CD133 Antibody (3Y4A5)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CD133

Prominin 1 (CD133) is expressed on primitive hematopoietic stem and progenitor cells, retinoblastoma, hemangioblasts, and neural stem cells as well as on developing epithelium. The CD133 positive fraction of human bone marrow, cord blood and peripheral blood have been shown to efficiently engraft in xenotransplantation models, and have been shown to contain the majority of the granulocyte/macrophage precursors, NOD/SCID repopulating cells and CD34 + dendritic cell precursors.

Alternate Names

AC133, CD133, PROM1, Prominin 1, PROML1

Gene Symbol

PROM1

Additional CD133 Products

Product Documents for CD133 Antibody (3Y4A5)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CD133 Antibody (3Y4A5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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