CD15/Lewis X Antibody (PD00-42)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32126
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human
Applications
Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # PD00-42
Concentration
1 mg/ml
Product Summary for CD15/Lewis X Antibody (PD00-42)
Immunogen
Purified CD15/Lewis X.
Localization
Golgi apparatus, Golgi stack membrane
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
59 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for CD15/Lewis X Antibody (PD00-42)
Immunohistochemistry: CD15/Lewis X Antibody (PD00-42) [NBP3-32126]
Immunohistochemistry: CD15/Lewis X Antibody (PD00-42) [NBP3-32126] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-CD15/Lewis X antibody (NBP3-32126) at 1/1,000 dilution.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32126) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Flow Cytometry: CD15/Lewis X Antibody (PD00-42) [NBP3-32126]
Flow Cytometry: CD15/Lewis X Antibody (PD00-42) [NBP3-32126] - Flow cytometry analysis of human peripheral blood granulocytes labelling CD15/Lewis X (NBP3-32126).Applications for CD15/Lewis X Antibody (PD00-42)
Application
Recommended Usage
Flow Cytometry
1:1000
Immunohistochemistry-Paraffin
1:1000
Application Notes
Recommended dilution for Multiplex IHC: 1:500
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH7.4), 0.1% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: CD15/Lewis X
SSEA1 antibodies are useful tools for differentiation studies and FACS analysis.
Alternate Names
CD15, Lewis-X, LeX, SSEA-1
Gene Symbol
FUT4
Additional CD15/Lewis X Products
Product Documents for CD15/Lewis X Antibody (PD00-42)
Product Specific Notices for CD15/Lewis X Antibody (PD00-42)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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