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CD151 Antibody (11G5a)

Novus Biologicals, part of Bio-Techne | Catalog # NB100-64810

Clone 11G5a was used by HLDA to establish CD designation.
Novus Biologicals, part of Bio-Techne
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NB100-64810
NB100-64810-0.025mg
Conjugate
Catalog #

Key Product Details

Species Reactivity

Human

Applications

ELISA, Flow Cytometry, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 11G5a

Concentration

1.0 mg/ml

Product Specifications

Immunogen

Made to Human CD151

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for CD151 Antibody (11G5a)

Flow Cytometry: CD151 Antibody (11G5a) - BSA Free [NB100-64810]

Flow Cytometry: CD151 Antibody (11G5a) - BSA Free [NB100-64810]

Flow Cytometry: CD151 Antibody (11G5a) [NB100-64810] - Staining of human peripheral blood platelets with MOUSE ANTI HUMAN CD151.

Applications for CD151 Antibody (11G5a)

Application
Recommended Usage

ELISA

1:100-1:2000

Flow Cytometry

1:50-1:100

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunohistochemistry-Paraffin

1:100

Immunoprecipitation

1:10-1:500

Western Blot

1:100-1:2000
Application Notes
For IHC: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose. For Flow Cytometry: Use 10ul of the suggested working dilution to label 10^6 cells or 100 ul whole blood. Western Blot: Clone 11G5a recognises human CD151 under non-reducing conditions.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD151

CD151 is essential for the proper assembly of the glomerular and tubular basement membranes in kidney. It interacts with integrins alpha3beta1, alpha5beta1, alpha3beta1 and alpha6beta4, and with CD9 and CD181. It is expressed in a variety of tissues including vascular endothelium and epidermis, platelets, monocytes and also erythroid cells, with a higher level of expression in erythroid precursors than on mature erythrocytes. CD151 is however not expressed by lymphocytes or granulocytes. Defects in CD151 are the cause of nephropathy with pretibial epidermolysis bullosa and deafness.

Alternate Names

CD151, PETA-3, SFA-1, TSPAN24

Entrez Gene IDs

977 (Human)

Gene Symbol

CD151

UniProt

Additional CD151 Products

Product Documents for CD151 Antibody (11G5a)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CD151 Antibody (11G5a)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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