CD163 Antibody (ED2)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-39099

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Rat

Cited:

Human, Rat

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # ED2

Concentration

0.5 mg/ml

View all CD163 Antibodies »

Product Specifications

Immunogen

Rat spleen cell homogenate

Reactivity Notes

Use in Human reported in scientific literature (PMID:34224892)

Specificity

This antibody recognizes the rat ED2 cell surface glycoprotein.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for CD163 Antibody (ED2)

Immunocytochemistry/ Immunofluorescence: CD163 Antibody (ED2) - BSA Free [NBP2-39099]

Immunocytochemistry/ Immunofluorescence: CD163 Antibody (ED2) - BSA Free [NBP2-39099]

Immunocytochemistry/Immunofluorescence: CD163 Antibody (ED2) [NBP2-39099] - Staining of rat lymph node cryosection. Image A in Red (NBP2-39099), and Image B in green using Mouse anti-Rat CD8. Image C is the merged image with nuclei counter-stained blue using DAPI. High power.
Immunohistochemistry-Paraffin: CD163 Antibody (ED2) - BSA Free [NBP2-39099]

Immunohistochemistry-Paraffin: CD163 Antibody (ED2) - BSA Free [NBP2-39099]

Immunohistochemistry-Paraffin: CD163 Antibody (ED2) - BSA Free [NBP2-39099] - Alcohol-fixed paraffin embedded spleen tissue from healthy rat spleen. Primary antibody dilution: 1:100. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: CD163 Antibody (ED2) - BSA Free [NBP2-39099]

Immunocytochemistry/ Immunofluorescence: CD163 Antibody (ED2) - BSA Free [NBP2-39099]

Immunocytochemistry/Immunofluorescence: CD163 Antibody (ED2) [NBP2-39099] - Staining of rat lymph node cryosection. Image A in Red (NBP2-39099), and Image B in green using Mouse anti-Rat CD8. Image C is the merged image with nuclei counter-stained blue using DAPI. Low power.
View 3 more images

Applications for CD163 Antibody (ED2)

Application
Recommended Usage

Flow Cytometry

1:10 - 1:100

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:50 - 1:100

Immunohistochemistry-Frozen

1:50 - 1:100

Immunohistochemistry-Paraffin

1:50 - 1:100

Immunoprecipitation

1:10 - 1:500

Western Blot

1:100 - 1:2000
Application Notes
This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase.
This antibody may be used in immunohistology using antigen retrieval, and has also been described reacting with paraffin-embedded material following PLP fixation (Periodate-lysine-paraformaldehyde). See Whiteland et al.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using NBP2-39099 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD163

CD163 (Cluster of Differentiation 163), also known by several other names including M130, p155, RM3/1, Ki-M8, Ber-MAC3, SM4, and GHI/61, is a type I transmembrane glycoprotein that is a member of the scavenger receptor cysteine-rich (SRCR) super family class B (1-3). CD163 is expressed specifically on the monocyte/macrophage lineage and has a theoretical molecular weight of 130-160 kDa in reducing conditions and 110 kDa in non-reducing conditions (2 - 6). CD163 is synthesized as 1076 amino acid (aa) protein consisting of a large extracellular domain (ECD) with nine SRCR domains, proline-serine-threonine rich (PST) linker domain, a transmembrane segment, and a cytoplasmic tail which has varying lengths depending on the isoform due to alternative splicing, with the short 49 aa form being the most common (1-3, 6).

One of the primary functions of CD163 is uptake of haptoglobin-hemoglobin (Hp-Hb) complexes from the liver, spleen, and bone marrow, ultimately triggering an anti-inflammatory response (3, 5, 7). CD163 also functions as an erythroblast adhesion receptor and promotes cell maturation and survival (3, 5, 7). Furthermore, CD163 functions in immune sensing of bacteria and as a receptor for tumor necrosis factor (TNF)-like weak inducer of apoptosis (TWEAK) (3, 5, 7). As mentioned above, CD163 is expressed on cells in the monocyte/macrophage lineage and, in general, anti-inflammatory signals including glucocorticoids, interleukin (IL)-6, and IL-10 stimulate CD163 synthesis and expression while, conversely, pro-inflammatory signals such as interferon-gamma (INF-gamma), TNF-alpha, and lipopolysaccharide (LPS) downregulate CD163 (3, 5). In addition to membrane-bound form of CD163, the protein can be cleaved by metalloproteinases (MMP) and induced by LPS or phorbol myristate acetate (PMA) to release a soluble form (sCD163) into the plasma (7). Increased levels of sCD163 in the plasma and an increased number of CD163-expressing macrophages at the site of inflammation are associated with a variety of pathologies (3, 5-7). CD163/sCD163 is often increased and a suitable clinical marker for inflammatory diseases including rheumatoid arthritis (RA), Gaucher disease, chronic kidney disease, diabetes, and Crohn's disease (3, 5-7).

Alternative names for CD163 includes GHI/61, HbSR, Hemoglobin scavenger receptor, M130, macrophage-associated antigen, MM130, RM3/1, SCARI1, scavenger receptor cysteine-rich type 1 protein M130, sCD163, and soluble CD163.

References

1. Law, S. K., Micklem, K. J., Shaw, J. M., Zhang, X. P., Dong, Y., Willis, A. C., & Mason, D. Y. (1993). A new macrophage differentiation antigen which is a member of the scavenger receptor superfamily. European journal of immunology. https://doi.org/10.1002/eji.1830230940

2. Onofre, G., Kolackova, M., Jankovicova, K., & Krejsek, J. (2009). Scavenger receptor CD163 and its biological functions. Acta medica (Hradec Kralove).

3. Van Gorp, H., Delputte, P. L., & Nauwynck, H. J. (2010). Scavenger receptor CD163, a Jack-of-all-trades and potential target for cell-directed therapy. Molecular immunology. https://doi.org/10.1016/j.molimm.2010.02.008

4. Sulahian, T. H., Hogger, P., Wahner, A. E., Wardwell, K., Goulding, N. J., Sorg, C., Droste, A., Stehling, M., Wallace, P. K., Morganelli, P. M., & Guyre, P. M. (2000). Human monocytes express CD163, which is upregulated by IL-10 and identical to p155. Cytokine. https://doi.org/10.1006/cyto.2000.0720

5. Etzerodt, A., & Moestrup, S. K. (2013). CD163 and inflammation: biological, diagnostic, and therapeutic aspects. Antioxidants & redox signaling. https://doi.org/10.1089/ars.2012.4834

6. Skytthe, M. K., Graversen, J. H., & Moestrup, S. K. (2020). Targeting of CD163+ Macrophages in Inflammatory and Malignant Diseases. International journal of molecular sciences, 21(15), 5497. https://doi.org/10.3390/ijms21155497

7. Moller H. J. (2012). Soluble CD163. Scandinavian journal of clinical and laboratory investigation. https://doi.org/10.3109/00365513.2011.626868

Alternate Names

CD163, GHI/61, HbSR, M130, RM3/1

Gene Symbol

CD163

UniProt

Q86VB7

Additional CD163 Products

Product Documents for CD163 Antibody (ED2)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for CD163 Antibody (ED2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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