Key Product Details

Species Reactivity

Validated:

Bovine

Applications

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # CC20

Concentration

1.0 mg/ml

View all CD1b Antibodies »

Product Summary for CD1b Antibody (CC20)

Immunogen

Made to Bovine CD1w2

Reactivity Notes

Reacts with Bovine. Cross reacts with Sheep, Goat, Dog and Horse.

Specificity

Recognizes the bovine CD1w2 cell surface antigen, a glycoprotein heterodimer of 12kD and 46kD. CD1w2 is expressed by cortical thymocytes and a minority of medullary thymocytes, with a pattern similar to antibodies of the CD1b cluster in humans.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for CD1b Antibody (CC20)

Immunohistochemistry: CD1b Antibody (CC20) - BSA Free [NB100-65315]

Immunohistochemistry: CD1b Antibody (CC20) - BSA Free [NB100-65315]

Immunohistochemistry: CD1b Antibody (CC20) [NB100-65315] - Detection of Mouse anti Bovine CD1w2 in an analysis on acetone-fixed frozen bovine thymus using the Histar detection system, original magnification x60.
Flow Cytometry: CD1b Antibody (CC20) - BSA Free [NB100-65315]

Flow Cytometry: CD1b Antibody (CC20) - BSA Free [NB100-65315]

Flow Cytometry: CD1b Antibody (CC20) [NB100-65315] - Figure A. FITC conjugated Mouse anti Bovine MHC Class II Monomorphic Antibody and Mouse IgG2a isotype control conjugated with Rabbit F(ab')2 anti Mouse IgG:RPE Antibody. Figure B. FITC conjugated Mouse anti Bovine MHC Class II Monomorphic Antibody and Mouse anti Bovine CD1w2 Antibody conjugated with Rabbit F(ab')2 anti Mouse IgG:RPE Antibody.
Flow Cytometry: CD1b Antibody (CC20) - BSA Free [NB100-65315]

Flow Cytometry: CD1b Antibody (CC20) - BSA Free [NB100-65315]

Flow Cytometry: CD1b Antibody (CC20) [NB100-65315] - Figure A. FITC conjugated Mouse anti Bovine MHC Class II Monomorphic Antibody and RPE conjugated Mouse IgG2a isotype control. Figure B. FITC conjugated Mouse anti Bovine MHC Class II Monomorphic Antibody and RPE conjugated Mouse anti Bovine CD1w2 Antibody. All experiments performed on bovine thymocytes in the presence of 10% bovine serum and gated on live, single cell thymocytes.
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Applications for CD1b Antibody (CC20)

Application
Recommended Usage

Flow Cytometry

1:25-1:50

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunoprecipitation

1:10-1:500
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD1b

All CD1 molecules, except CD1e, are cell surface glycoproteins that are structurally related to the MHC molecules, however, in distinction, CD1 proteins are essentially non polymorphic. CD1 has considerable structural homology with both MHC class I and class II molecules, and CD1 molecules are involved in T cell activation. In contrast to MHC, however, CD1 molecules appear to present predominantly non peptide molecules originating from lipids and glycolipids.

Alternate Names

CD1b

Gene Symbol

CD1B

Additional CD1b Products

Product Documents for CD1b Antibody (CC20)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for CD1b Antibody (CC20)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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