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CD1b Antibody (RIV12) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34714

Novus Biologicals, part of Bio-Techne
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NBP2-34714-0.1mg

Key Product Details

Species Reactivity

Validated:

Human

Applications

CyTOF-ready, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # RIV12

Format

Azide and BSA Free

Concentration

1.0 mg/ml

Product Summary for CD1b Antibody (RIV12) - Azide and BSA Free

Immunogen

Human peripheral lymphocytes (Uniprot: P29016)

Localization

Cell surface

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

49 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-32925).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for CD1b Antibody (RIV12) - Azide and BSA Free

Flow Cytometry: CD1b Antibody (RIV12) - Azide and BSA Free [NBP2-34714]

Flow Cytometry: CD1b Antibody (RIV12) - Azide and BSA Free [NBP2-34714]

Flow Cytometry: CD1b Antibody (RIV12) - Azide and BSA Free [NBP2-34714] - FCM staining of human PBMCs using CD1b MAb (RIV12).

Applications for CD1b Antibody (RIV12) - Azide and BSA Free

Application
Recommended Usage

ELISA

1-5ug/ml for coating

Flow Cytometry

0.5-1ug/million cells

Immunocytochemistry/ Immunofluorescence

0.5-1ug/ml
Application Notes
ELISA: For coating, order antibody without BSA).
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: CD1b

All CD1 molecules, except CD1e, are cell surface glycoproteins that are structurally related to the MHC molecules, however, in distinction, CD1 proteins are essentially non polymorphic. CD1 has considerable structural homology with both MHC class I and class II molecules, and CD1 molecules are involved in T cell activation. In contrast to MHC, however, CD1 molecules appear to present predominantly non peptide molecules originating from lipids and glycolipids.

Alternate Names

CD1b

Gene Symbol

CD1B

Additional CD1b Products

Product Documents for CD1b Antibody (RIV12) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CD1b Antibody (RIV12) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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