Flow Cytometry: CD1d Antibody (51.1) [NBP1-43460] - Staining of normal human peripheral blood cells with 0.5 ug of Mouse IgG2b K Isotype Control Purified (open histogram) or Anti-Human CD1d Purified (filled histogram) followed by Anti-Mouse IgG Biotin and Streptavidin PE . Cells in the lymphocyte gate were

Applications for CD1d Antibody (51.1) - BSA Free

Application

Recommended Usage

Flow Cytometry

1:10-1:1000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunoprecipitation

1:10-1:500

Application Notes

This 51.1 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.5 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test.

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS (pH 7.2)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CD1d

The monoclonal antibody 51.1 reacts with human CD1d, a member of the CD1 family with similarity to the non-polymorphic MHC Class I-like molecules. CD1d is a highly conserved single transmembrane receptor of the Immunoglobulin Superfamily. CD1d can associate with beta-microglobulin another feature showing similarity to MHC class I molecules, but can also exist as a nonglycosylated protein not in association with beta microglobulin. This suggests different control mechanisms for presenting glycolipid containing molecules to CD1d reactive NKT cells. Expression of CD1d is found on B cells of the periphery, in resting monocytes and cortical thymocytes. On intestinal epithelial cells (IEC) expression is polarized. Expression can also be found at low levels intracellularly in hepatocytes. In HCV (hepatitis C virus) livers, CD1d is highly expressed compared to normal controls.

Alternate Names

CD1d, R3, R3G1

Gene Symbol

CD1D

Additional CD1d Products

Product Documents for CD1d Antibody (51.1) - BSA Free

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for CD1d Antibody (51.1) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations
Reviews

CD1d Antibody (51.1) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-43460

Key Product Details

Species Reactivity

Validated:

Applications

Label

Antibody Source

Format

Concentration

View all CD1d Antibodies »

Product Summary for CD1d Antibody (51.1) - BSA Free

Immunogen

Clonality

Host

Isotype

Scientific Data Images for CD1d Antibody (51.1) - BSA Free

Flow Cytometry: CD1d Antibody (51.1) - BSA Free [NBP1-43460]

Applications for CD1d Antibody (51.1) - BSA Free

Flow Cytometry

Immunohistochemistry

Immunohistochemistry-Frozen

Immunoprecipitation

Formulation, Preparation, and Storage

Purification

Formulation

Format

Preservative

Concentration

Shipping

Stability & Storage

Background: CD1d

Alternate Names

Gene Symbol

Additional CD1d Products

Product Documents for CD1d Antibody (51.1) - BSA Free

Product Datasheets

COA

Certificate of Analysis

SDS

Product Specific Notices for CD1d Antibody (51.1) - BSA Free