CD21 Antibody (Bu33)
Novus Biologicals, part of Bio-Techne | Catalog # NB100-63574
Clone Bu33 was used by HLDA to establish CD designation.
![Flow Cytometry: CD21 Antibody (Bu33) - BSA Free [NB100-63574]](https://resources.bio-techne.com/images/products/CD21-Antibody-Bu33-Flow-Cytometry-NB100-63574-img0003.jpg "Flow Cytometry: CD21 Antibody (Bu33) - BSA Free [NB100-63574]")
![Flow Cytometry: CD21 Antibody (Bu33) - BSA Free [NB100-63574]](https://resources.bio-techne.com/images/products/CD21-Antibody-Bu33-Flow-Cytometry-NB100-63574-img0004.jpg "Flow Cytometry: CD21 Antibody (Bu33) - BSA Free [NB100-63574]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # Bu33
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Made to Human CD21
Specificity
NB100-63574 recognizes human CD21, a 145kD cell surface glycoprotein expressed by mature B lymphocytes, follicular dendritic cells, subsets of normal thymocytes and subsets of T cells. The CD21 molecule acts as a receptor for CD23, for complement components C3d, C3dg and iC3b and for Epstein Barr Virus. CD21 is part of the signalling complex that is linked to CD19, CD81 and phosphatidylinositol-3-kinase.
Marker
Histiocytic Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for CD21 Antibody (Bu33)
Flow Cytometry: CD21 Antibody (Bu33) - BSA Free [NB100-63574]
Flow Cytometry: CD21 Antibody (Bu33) [NB100-63574] - Flow Cytometry: CD21 Antibody (Bu33) [Alexa Fluor 647] [NB100-63574AF647] - A surface stain was performed on human peripheral blood lymphocytes with CD21 (Bu33) antibody NB100-63574AF647 and a matched isotype control NBP2-27287AF647. Cells were incubated in an antibody dilution of 2 ug/mL for 20 minutes at room temperature. A co-stain was performed with NBP2-26646. Image using the Alexa Fluor 647 form of this antibody.![Flow Cytometry: CD21 Antibody (Bu33) - BSA Free [NB100-63574]](https://resources.bio-techne.com/images/products/CD21-Antibody-Bu33-Flow-Cytometry-NB100-63574-img0001.jpg "Flow Cytometry: CD21 Antibody (Bu33) - BSA Free [NB100-63574]")
Flow Cytometry: CD21 Antibody (Bu33) - BSA Free [NB100-63574]
Flow Cytometry: CD21 Antibody (Bu33) [NB100-63574] - Using the Alexa Fluor 488 direct conjugate A surface stain was performed on human peripheral blood lymphocytes with CD21 (Bu33) antibody NB100-63574AF488 and a matched isotype control. Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. A co-stain was performed with NBP2-25196AF647.![Flow Cytometry: CD21 Antibody (Bu33) - BSA Free [NB100-63574]](https://resources.bio-techne.com/images/products/CD21-Antibody-Bu33-Flow-Cytometry-NB100-63574-img0002.jpg "Flow Cytometry: CD21 Antibody (Bu33) - BSA Free [NB100-63574]")
Flow Cytometry: CD21 Antibody (Bu33) - BSA Free [NB100-63574]
Flow Cytometry: CD21 Antibody (Bu33) [NB100-63574] - Using the Allophycocyanin direct conjugate A cell surface stain was performed on Daudi cells with CD21 Antibody (Bu33) NB100-63574APC (blue) and a matched isotype control NBP2-27287APC (orange). Cells were incubated in an antibody dilution of 0.5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to allophycocyanin.Applications for CD21 Antibody (Bu33)
Application
Recommended Usage
Flow Cytometry
1:50-1:200
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: CD21
Additional CD21 Products
Product Documents for CD21 Antibody (Bu33)
Product Specific Notices for CD21 Antibody (Bu33)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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