CD21 Antibody (Bu33)

Novus Biologicals, part of Bio-Techne | Catalog # NB100-63574

Clone Bu33 was used by HLDA to establish CD designation.
Novus Biologicals, part of Bio-Techne
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NB100-63574-0.025mg
NB100-63574
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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # Bu33

Concentration

1.0 mg/ml

View all CD21 Antibodies »

Product Specifications

Immunogen

Made to Human CD21

Reactivity Notes

Predicted cross-reactivities: Equine

Specificity

NB100-63574 recognizes human CD21, a 145kD cell surface glycoprotein expressed by mature B lymphocytes, follicular dendritic cells, subsets of normal thymocytes and subsets of T cells. The CD21 molecule acts as a receptor for CD23, for complement components C3d, C3dg and iC3b and for Epstein Barr Virus. CD21 is part of the signalling complex that is linked to CD19, CD81 and phosphatidylinositol-3-kinase.

Marker

Histiocytic Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for CD21 Antibody (Bu33)

Flow Cytometry: CD21 Antibody (Bu33) - BSA Free [NB100-63574]

Flow Cytometry: CD21 Antibody (Bu33) - BSA Free [NB100-63574]

Flow Cytometry: CD21 Antibody (Bu33) [NB100-63574] - Flow Cytometry: CD21 Antibody (Bu33) [Alexa Fluor 647] [NB100-63574AF647] - A surface stain was performed on human peripheral blood lymphocytes with CD21 (Bu33) antibody NB100-63574AF647 and a matched isotype control NBP2-27287AF647. Cells were incubated in an antibody dilution of 2 ug/mL for 20 minutes at room temperature. A co-stain was performed with NBP2-26646. Image using the Alexa Fluor 647 form of this antibody.
Flow Cytometry: CD21 Antibody (Bu33) - BSA Free [NB100-63574]

Flow Cytometry: CD21 Antibody (Bu33) - BSA Free [NB100-63574]

Flow Cytometry: CD21 Antibody (Bu33) [NB100-63574] - Using the Alexa Fluor 488 direct conjugate A surface stain was performed on human peripheral blood lymphocytes with CD21 (Bu33) antibody NB100-63574AF488 and a matched isotype control. Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. A co-stain was performed with NBP2-25196AF647.
Flow Cytometry: CD21 Antibody (Bu33) - BSA Free [NB100-63574]

Flow Cytometry: CD21 Antibody (Bu33) - BSA Free [NB100-63574]

Flow Cytometry: CD21 Antibody (Bu33) [NB100-63574] - Using the Allophycocyanin direct conjugate A cell surface stain was performed on Daudi cells with CD21 Antibody (Bu33) NB100-63574APC (blue) and a matched isotype control NBP2-27287APC (orange). Cells were incubated in an antibody dilution of 0.5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to allophycocyanin.
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Applications for CD21 Antibody (Bu33)

Application
Recommended Usage

Flow Cytometry

1:50-1:200

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD21

CD21 (complement receptor 2, CR2) binds C3 complement fragments, especially its breakdown fragments, which remain covalently attached to complement activating surfaces or antigen. CD21 has important roles in uptake and retention of immunocomplexes, survival of memory B cells and in development and maintenance of the humoral response to T-dependent antigens. CD21 also serves as a key receptor for Epstein-Barr virus binding and is involved in targeting prions to folicular dendritic cells and expediting neuroinvasion following peripheral exposure to prions. A soluble form of the CD21 (sCD21) is shed from the lymphocyte surface and retains its ability to bind respective ligands.

Alternate Names

C3DR, CD21, CR2

Gene Symbol

CR2

UniProt

P20023 (Human)

Additional CD21 Products

Product Documents for CD21 Antibody (Bu33)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for CD21 Antibody (Bu33)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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