CD21 Antibody (CR2/3124R)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07754
Recombinant Monoclonal Antibody
![Immunocytochemistry/ Immunofluorescence: CD21 Antibody (CR2/3124R) [NBP3-07754]](https://resources.bio-techne.com/images/products/CD21-Antibody-CR2-3124R-Immunocytochemistry-Immunofluorescence-NBP3-07754-img0002.jpg "Immunocytochemistry/ Immunofluorescence: CD21 Antibody (CR2/3124R) [NBP3-07754]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # CR2/3124R
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Recombinant fragment (around aa 142-240) of human CR2 (CD21) protein (exact sequence is proprietary) (Uniprot: P20023)
Localization
Cell Surface
Marker
Mature B-Cell & Follicular Dendritic Cell Marker
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
140 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08297)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for CD21 Antibody (CR2/3124R)
Immunocytochemistry/ Immunofluorescence: CD21 Antibody (CR2/3124R) [NBP3-07754]
Immunocytochemistry/Immunofluorescence: CD21 Antibody (CR2/3124R) [NBP3-07754] - Immunofluorescence staining of PFA-fixed MOLT4 cells using CD21 Recombinant Rabbit Monoclonal Antibody (CR2/3124R) followed by goat anti- Mouse IgG-CF488 (green). Nuclei are stained with RedDot.![Immunohistochemistry-Paraffin: CD21 Antibody (CR2/3124R) [NBP3-07754]](https://resources.bio-techne.com/images/products/CD21-Antibody-CR2-3124R-Immunohistochemistry-Paraffin-NBP3-07754-img0003.jpg "Immunohistochemistry-Paraffin: CD21 Antibody (CR2/3124R) [NBP3-07754]")
Immunohistochemistry-Paraffin: CD21 Antibody (CR2/3124R) [NBP3-07754]
Immunohistochemistry-Paraffin: CD21 Antibody (CR2/3124R) [NBP3-07754] - Formalin-fixed, paraffin-embedded human Tonsil Dendritic stained with CD21 Recombinant Rabbit Monoclonal Antibody (CR2/3124R).![Flow Cytometry: CD21 Antibody (CR2/3124R) [NBP3-07754]](https://resources.bio-techne.com/images/products/CD21-Antibody-CR2-3124R-Flow-Cytometry-NBP3-07754-img0001.jpg "Flow Cytometry: CD21 Antibody (CR2/3124R) [NBP3-07754]")
Flow Cytometry: CD21 Antibody (CR2/3124R) [NBP3-07754]
Flow Cytometry: CD21 Antibody (CR2/3124R) [NBP3-07754] - Flow Cytometric Analysis of PFA-fixed MOLT4 cells. CD21 Recombinant Rabbit Monoclonal Antibody (CR2/3124R) followed by Goat anti-rabbit IgG-CF488 (Blue); Isotype Control (Red).Applications for CD21 Antibody (CR2/3124R)
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Western Blot
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: CD21
Additional CD21 Products
Product Documents for CD21 Antibody (CR2/3124R)
Product Specific Notices for CD21 Antibody (CR2/3124R)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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