CD3 zeta Antibody (1T5R7)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15349

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15349-100ul
NBP3-15349-20ul
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1T5R7

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CD3 zeta/CD247 Antibodies »

Product Summary for CD3 zeta Antibody (1T5R7)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 65-164 of human CD3 zeta (P20963). QQGQNQLYNELNLGRREEYDVLDKRRGRDPEMGGKPQRRKNPQEGLYNELQKDKMAEAYSEIGMKGERRRGKGHDGLYQGLSTATKDTYDALHMQALPPR

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CD3 zeta Antibody (1T5R7)

Western Blot: CD3 zeta Antibody (1T5R7) [NBP3-15349]

Western Blot: CD3 zeta Antibody (1T5R7) [NBP3-15349]

Western Blot: CD3 zeta Antibody (1T5R7) [NBP3-15349] - Western blot analysis of extracts of Jurkat cells, using CD3 zeta Rabbit mAb (NBP3-15349) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.
Immunohistochemistry-Paraffin: CD3 zeta Antibody (1T5R7) [NBP3-15349]

Immunohistochemistry-Paraffin: CD3 zeta Antibody (1T5R7) [NBP3-15349]

Immunohistochemistry-Paraffin: CD3 zeta Antibody (1T5R7) [NBP3-15349] - Immunohistochemistry of paraffin-embedded human colon using CD3 zeta Rabbit mAb (NBP3-15349) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Flow Cytometry: CD3 zeta Antibody (1T5R7) [NBP3-15349]

Flow Cytometry: CD3 zeta Antibody (1T5R7) [NBP3-15349]

Flow Cytometry: CD3 zeta Antibody (1T5R7) [NBP3-15349] - Flow cytometry: Jurkat cells were stained with Rabbit IgG isotype control (AC042, 10 ug/ml, blue line) or CD3 zeta Rabbit mAb (NBP3-15349, 10 ug/ml orange line), followed by goat anti-Rabbit pAb FITC (1:200 dilution) staining. Non-fluorescently stained Jurkat cells was used as blank control (red line) .
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Applications for CD3 zeta Antibody (1T5R7)

Application
Recommended Usage

Flow Cytometry

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CD3 zeta/CD247

CD3 (Cluster of Differentiation 3) is a complex of proteins that associates directly with the T cell antigen receptor (TCR). Antigen binding to the TCR leads to IL-2 secretion via activation of a tyrosine phosphorylation pathway and a phospholipase C (PLC) pathway, in turn activating protein kinase C. CD3 is composed of five invariant polypeptide chains that associate to form three dimers. The five invariant chains of CD3 are labeled gamma, delta, epsilon, zeta, and eta. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. Loss of the zeta chain results in the synthesis of unstable TCRs. A decrease of CD3 zeta has been described in T cells from patients with cancer, lupus and chronic infectious diseases.

Long Name

T-cell Receptor zeta

Alternate Names

CD247, CD3H, CD3Q, CD3Z, IMD25, T3Z, TCR Zeta Chain

Gene Symbol

CD247

Additional CD3 zeta/CD247 Products

Product Documents for CD3 zeta Antibody (1T5R7)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CD3 zeta Antibody (1T5R7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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