CD35 Antibody (8C12) [PE/Cy5.5] - Chimeric

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-52668PECY55

Recombinant Monoclonal Antibody.
Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry

Label

PE/Cy5.5 (Excitation = 488 nm, Emission = 695 nm)

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # 8C12

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CD35 Antibodies »

Product Specifications

Immunogen

Purified murine CR1 protein.

Reactivity Notes

Mouse

Specificity

Recognises an epitope on CD35 (CR1, 190 kDa), but not on CD21 (CR2, 145-150 kDa), which are alternative splice variants of primary Cr2 gene transcripts.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Applications for CD35 Antibody (8C12) [PE/Cy5.5] - Chimeric

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined. For optimal results using our Tandem dyes, please avoid prolonged exposure to light or extreme temperature fluctuations. These can lead to irreversible degradation or decoupling. When staining intracellular targets, specific attention to the fixation and permeabilization steps in your flow protocol may be required. Please contact our technical support team at technical@novusbio.com if you have any questions.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark. Do not freeze.

Background: CD35

CD35 encodes a membrane glycoprotein found on peripheral blood cells, glomerular podocytes, and follicular dendritic cells. The protein is a receptor for complement components C3b and C4b and regulates the activity of the complement cascade. Variation in this protein is the basis of the Knops blood group system. The two most common alleles, F and S, differ by 8 exons and are thought to be the result of an unequal crossover event. A secreted form of the protein present in plasma has been described, but its full length nature has not been determined.

Alternate Names

C3BR, CD35, CR1, KN

Gene Symbol

CR1

Additional CD35 Products

Product Documents for CD35 Antibody (8C12) [PE/Cy5.5] - Chimeric

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CD35 Antibody (8C12) [PE/Cy5.5] - Chimeric

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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