CD40 Ligand/TNFSF5 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-91770

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all CD40 Ligand/TNFSF5 Antibodies »

Product Summary for CD40 Ligand/TNFSF5 Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LTVKRQGLYYIYAQVTFCSNREASSQAPFIASLCLKSPGRFERILLRAANTHSSAKPCGQQSIHLGGVFELQPGASVFVNVTDPSQVS

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CD40 Ligand/TNFSF5 Antibody

Western Blot: CD40 Ligand/TNFSF5 Antibody [NBP1-91770]

Western Blot: CD40 Ligand/TNFSF5 Antibody [NBP1-91770]

Western Blot: CD40 Ligand/TNFSF5 Antibody [NBP1-91770] - Analysis in control (vector only transfected HEK293T lysate) and cD40LG over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells.
Immunohistochemistry-Paraffin: CD40 Ligand/TNFSF5 Antibody [NBP1-91770]

Immunohistochemistry-Paraffin: CD40 Ligand/TNFSF5 Antibody [NBP1-91770]

Immunohistochemistry-Paraffin: CD40 Ligand/TNFSF5 Antibody [NBP1-91770] - Staining of human rectum shows strong cytoplasmic positivity in leukocytes.
Immunohistochemistry-Paraffin: CD40 Ligand/TNFSF5 Antibody [NBP1-91770]

Immunohistochemistry-Paraffin: CD40 Ligand/TNFSF5 Antibody [NBP1-91770]

Immunohistochemistry-Paraffin: CD40 Ligand/TNFSF5 Antibody [NBP1-91770] - Staining of human cerebral cortex shows no positivity in neurons as expected.
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Applications for CD40 Ligand/TNFSF5 Antibody

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD40 Ligand/TNFSF5

CD40L is the ligand for CD40, a member of the tumor necrosis factor (TNF) receptor superfamily. CD40L is expressed mainly on activated CD4+ T-lymphocytes as a single-pass type II membrane protein. Proteolytic processing of the membrane form results in a soluble secreted form of CD40L.

Alternate Names

CD154, CD40L, CD40LG, gp39, TNFSF5, TRAP

Gene Symbol

CD40LG

Additional CD40 Ligand/TNFSF5 Products

Product Documents for CD40 Ligand/TNFSF5 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CD40 Ligand/TNFSF5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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