Key Product Details

Species Reactivity

Validated:

Human

Applications

Flow (Cell Surface), Flow Cytometry, Immunocytochemistry, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgM Kappa Clone # PAC-1

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CD41/CD61 Antibodies »

Product Summary for CD41/CD61 Antibody (PAC-1)

Immunogen

Human platelets

Specificity

The mouse monoclonal antibody PAC-1 recognizes an extracellular activation-induced conformational epitope PAC-1 on CD41/CD61 complex (gpIIb/IIIa), also known as integrin alpha IIb beta 3, a receptor which mediates platelet aggregation.

Clonality

Monoclonal

Host

Mouse

Isotype

IgM Kappa

Scientific Data Images for CD41/CD61 Antibody (PAC-1)

Flow Cytometry: CD41/CD61 Antibody (PAC-1) [NBP2-62201]

Flow Cytometry: CD41/CD61 Antibody (PAC-1) [NBP2-62201]

Flow Cytometry: CD41/CD61 Antibody (PAC-1) [NBP2-62201] - Separation of CD41/CD61 positive platelets (red-filled) from CD41/CD61 negative nucleated cells (black-dashed) in flow cytometry analysis (surface staining) of PHA stimulated human peripheral whole blood stained using anti-human CD41/CD61 (PAC-1) purified antibody (concentration in sample 8 ug/ml, GAM PE).
Flow (Cell Surface): CD41/CD61 Antibody (PAC-1) [NBP2-62201]

Flow (Cell Surface): CD41/CD61 Antibody (PAC-1) [NBP2-62201]

Flow (Cell Surface): CD41/CD61 Antibody (PAC-1) [NBP2-62201] - Surface staining of PAC-1 on PHA-activated (1 hour) human peripheral blood with anti-PAC-1 (PAC-1) FITC.
Flow (Cell Surface): CD41/CD61 Antibody (PAC-1) [NBP2-62201]

Flow (Cell Surface): CD41/CD61 Antibody (PAC-1) [NBP2-62201]

Flow (Cell Surface): CD41/CD61 Antibody (PAC-1) [NBP2-62201] - Surface staining pattern of PHA stimulated human peripheral whole blood stained using anti-human CD41/CD61 (PAC-1) purified antibody (concentration in sample 8 ug/ml, GAM PE).
View 1 more images

Applications for CD41/CD61 Antibody (PAC-1)

Application
Recommended Usage

Flow Cytometry

2-4 ug/ml

Immunoprecipitation

1:10-1:500
Application Notes
Purified from cell culture supernatant by PEG precipitation and hydroxyapatite chromatography.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Multi-step

Formulation

TBS (pH 8.0)

Preservative

0.9% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CD41/CD61

Integrin alpha 2b (CD41) is a calcium-dependent, noncovalently associated heterodimer and contains a heavy chain (GPIIb alpha) and a light chain (GPIIb beta) linked by a single disulfide bond. The Integrin alpha 2B chain interacts with the Integrin beta 3 subunit/CD61 to form the platelet glycoprotein complex, gpIIb/IIIa. It is expressed on platelets and megakaryocytes. Ligands for the gpIIb/IIIa heterodimer include fibrinogen, von Willebrand factor, fibronectin, vitronectin, and thrombospondin. The gpIIb/IIIa complex is the major Integrin on platelets and is important for platelet adhesion and aggregation.

Alternate Names

CD41, CD41 antigen, CD41BHPA3, GP2Bintegrin alpha-IIb, GPalpha IIb, GPIIb, GTA, integrin, alpha 2b (platelet glycoprotein IIb of IIb/IIIa complex, antigenCD41), integrin, alpha 2b (platelet glycoprotein IIb of IIb/IIIa complex, antigenCD41B), ITGAB, platelet fibrinogen receptor, alpha subunit, Platelet membrane glycoprotein IIb, platelet-specific antigen BAK

Gene Symbol

ITGA2B

Additional CD41/CD61 Products

Product Documents for CD41/CD61 Antibody (PAC-1)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CD41/CD61 Antibody (PAC-1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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