CD43/Sialophorin Antibody (R2/60)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-43413

Novus Biologicals, part of Bio-Techne
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NBP1-43413-0.1mg
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Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgM Clone # R2/60

Concentration

0.5 mg/ml

View all CD43 Antibodies »

Product Specifications

Immunogen

The immunogen for this antibody was CD43.

Reactivity Notes

This antibody is reactive to Mouse.

Clonality

Monoclonal

Host

Rat

Isotype

IgM

Scientific Data Images for CD43/Sialophorin Antibody (R2/60)

Flow Cytometry: CD43/Sialophorin Antibody (R2/60) [NBP1-43413]

Flow Cytometry: CD43/Sialophorin Antibody (R2/60) [NBP1-43413]

Flow Cytometry: CD43/Sialophorin Antibody (R2/60) [NBP1-43413] - Analysis using the FITC conjugate of NBP1-43413. Staining of C57BL/6 splenocytes with Anti-Human/Mouse CD45R (B220) APC and 0.06 ug of Rat IgM kappa Isotype Control FITC (left) or 0.06 ug of Anti-Mouse CD43 FITC (right).
Flow Cytometry: CD43/Sialophorin Antibody (R2/60) [NBP1-43413]

Flow Cytometry: CD43/Sialophorin Antibody (R2/60) [NBP1-43413]

Flow Cytometry: CD43/Sialophorin Antibody (R2/60) [NBP1-43413] - Staining of C57Bl/6 splenocytes with 0.25 ug of Rat IgM Isotype Control Purified (open histogram) or 0.25 ug of Anti-Mouse CD43 Purified (filled histogram) followed by Anti-Rat IgM PE . Cells in the lymphocyte gate were used for analysis.
Flow Cytometry: CD43/Sialophorin Antibody (R2/60) [NBP1-43413]

Flow Cytometry: CD43/Sialophorin Antibody (R2/60) [NBP1-43413]

Flow Cytometry: CD43/Sialophorin Antibody (R2/60) [NBP1-43413] - Analysis using the Biotin conjugate of NBP1-43413. Staining of C57BL/6 splenocytes with Anti-Human/Mouse CD45R (B220) APC and 0.25 ug of Rat IgM kappa Isotype Control Biotin (left) or 0.25 ug of Anti-Mouse CD43 Biotin (right) followed by Streptavidin PE.

Applications for CD43/Sialophorin Antibody (R2/60)

Application
Recommended Usage

Flow Cytometry

1:10-1:1000

Immunoprecipitation

1:10-1:500

Western Blot

1:100-1:2000
Application Notes
This R2/60 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2)

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CD43

The R2/60 monoclonal antibody reacts with mouse CD43, also known as leukosialin and sialophorin. CD43 has glycoforms of approximately 115 and 130 kDa, and is expressed by an early B cell subset in BM, thymocytes and mature T cells, NK cells, myeloid lineage cells and platelets. CD43 is reported to bind to CD54 and play a role in adhesion and B cell survival. While the S7 and 1B11 monoclonal antibody clones detect only certain isoforms of CD43, data suggests that R2/60 detects the expression of both isoforms. It has been demonstrated that R2/60 stains the majority of CD4+ and CD8+ splenocytes, as well as a significant population of CD19+ splenocytes. However, the clones S7 and 1B11 stain only CD4+ and CD8+, or CD19+ splenocytes, respectively. This suggests that S7 and 1B11 recognize distinct epitopes which are differentially expressed by splenic B and T cells, whereas R2/60 recognizes an epitope shared by both isoforms of CD43.

Long Name

Cluster of Differentiation 43

Alternate Names

CD43, GPL115, Leukosialin, LSN, Ly-48, Sialophorin, SPN

Gene Symbol

SPN

Additional CD43 Products

Product Documents for CD43/Sialophorin Antibody (R2/60)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CD43/Sialophorin Antibody (R2/60)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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