CD43/Sialophorin Antibody (SPM503) - IHC-Prediluted
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-44647
![Immunohistochemistry-Paraffin: CD43/Sialophorin Antibody (SPM503) - IHC-Prediluted [NBP2-44647]](https://resources.bio-techne.com/images/products/CD43-Sialophorin-Antibody-SPM503-IHC-Prediluted-Immunohistochemistry-Paraffin-NBP2-44647-img0002.jpg "Immunohistochemistry-Paraffin: CD43/Sialophorin Antibody (SPM503) - IHC-Prediluted [NBP2-44647]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # SPM503
Format
IHC-Prediluted
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for CD43/Sialophorin Antibody (SPM503) - IHC-Prediluted
Immunogen
Myeloblastic KG1 cells
Localization
Cell surface
Specificity
It recognizes a cell surface glycoprotein of 95/115/135kDa (depending upon the extent of glycosylation), identified as CD43. 70-90% of T-cell lymphomas and from 22-37% of B-cell lymphomas express CD43. No reactivity has been observed with reactive B-cells. So, a B-lineage population that co-expresses CD43 is highly likely to be a malignant lymphoma, especially a low-grade lymphoma, rather than a reactive B-cell population. When CD43 antibody is used in combination with anti-CD20, effective immunophenotyping of the lymphomas in formalin-fixed tissues can be obtained. Co-staining of a lymphoid infiltrate with anti-CD20 and anti-CD43 argues against a reactive process and favors a diagnosis of lymphoma.
Marker
T-Cell Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.
Scientific Data Images for CD43/Sialophorin Antibody (SPM503) - IHC-Prediluted
Immunohistochemistry-Paraffin: CD43/Sialophorin Antibody (SPM503) - IHC-Prediluted [NBP2-44647]
Immunohistochemistry-Paraffin: CD43/Sialophorin Antibody (SPM503) - IHC-Prediluted [NBP2-44647] - Formalin-fixed, paraffin-embedded human tonsil stained with CD43/Sialophorin Mouse Monoclonal Antibody (SPM503).Applications for CD43/Sialophorin Antibody (SPM503) - IHC-Prediluted
Application
Recommended Usage
Immunohistochemistry-Paraffin
0.5 - 1.0 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Format
IHC-Prediluted
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: CD43
Long Name
Cluster of Differentiation 43
Alternate Names
CD43, GPL115, Leukosialin, LSN, Ly-48, Sialophorin, SPN
Gene Symbol
SPN
Additional CD43 Products
Product Documents for CD43/Sialophorin Antibody (SPM503) - IHC-Prediluted
Product Specific Notices for CD43/Sialophorin Antibody (SPM503) - IHC-Prediluted
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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