CD45RA Antibody (OTH-74D4) - Chimeric - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-12063

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-12063-0.025mg
NBP3-12063-0.2mg
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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # OTH-74D4

Format

Azide and BSA Free

Concentration

1 mg/ml

View all CD45RA Antibodies »

Product Specifications

Immunogen

This antibody was raised by immunising mice with an EBV-transformed B cell line from a patient with MHC class II deficiency.

Specificity

OTH-74D4 antibody recognises CD45RA, which is an CD45 isoform containing only protein A region.The CD45RA antigen is expressed mostly on naive CD4+, CD8+ and gd+ T and on most B and NK lymphocytes. Generally the CD45RA antigen density decreases upon activation.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Scientific Data Images for CD45RA Antibody (OTH-74D4) - Chimeric - Azide and BSA Free

Flow Cytometry: CD45RA Antibody (OTH-74D4) - Chimeric - Azide and BSA Free [NBP3-12063]

Flow Cytometry: CD45RA Antibody (OTH-74D4) - Chimeric - Azide and BSA Free [NBP3-12063]

Flow Cytometry: CD45RA Antibody (OTH-74D4) - Chimeric [NBP3-12063] - Using the Anti-CD45RA antibody OTH-74D4 (Ab01502). Paraformaldehyde fixed Jurkat cells permeabilized with 0.5% Triton were stained with CD45RA antibody (Ab00178-23.0; isotype control, black line) or the rabbit IgG version of OTH-74D4 (Ab01502-23.0, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
Immunofluorescence: CD45RA Antibody (OTH-74D4) - Chimeric - Azide and BSA Free [NBP3-12063]

Immunofluorescence: CD45RA Antibody (OTH-74D4) - Chimeric - Azide and BSA Free [NBP3-12063]

Immunofluorescence: CD45RA Antibody (OTH-74D4) - Chimeric [NBP3-12063] - Staining of Jukat cells with anti-CD45RA (Ab01502) OTH-74D4 Immunofluorescence analysis of paraformaldehyde fixed Jukat cells on Shi-fix™ cover-slips stained with the chimeric rabbit IgG version of OTH-74D4 (Ab01502-23.0) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom Ab01502-23.0, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (Ab00178-23.0) followed by staining with Alexa Fluor® 488 secondary antibody.

Applications for CD45RA Antibody (OTH-74D4) - Chimeric - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.

Background: CD45RA

The CD45 molecule is comprised of different glycoproteins ranging from 180-240 kD. Expression of CD45 is found on all hemopoietic cells. Detection of the different isoforms can distinguish between different cell forms i.e. naive T cells and memory T cells. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. The functional activity of each CD45 isotype also varies.

Long Name

CD45R, A isoform

Alternate Names

B220, CD45 antigen, CD45R, EC 3.1.3.48, L-CA, LY5, protein tyrosine phosphatase, receptor type, C, receptor-type tyrosine-protein phosphatase C, T200 glycoprotein, T200 leukocyte common antigen, T200receptor type, c polypeptide

Gene Symbol

PTPRC

Additional CD45RA Products

Product Documents for CD45RA Antibody (OTH-74D4) - Chimeric - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CD45RA Antibody (OTH-74D4) - Chimeric - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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