CD45RB Antibody (BRA-11 (same as BRA-11G)) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34564
![Western Blot: CD45RB Antibody (BRA-11 (same as BRA-11G))Azide and BSA Free [NBP2-34564]](https://resources.bio-techne.com/images/products/CD45RB-Antibody-BRA-11-same-as-BRA-11G-Azide-and-BSA-Free-Western-Blot-NBP2-34564-img0001.jpg "Western Blot: CD45RB Antibody (BRA-11 (same as BRA-11G))Azide and BSA Free [NBP2-34564]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Monkey (Negative), Rat (Negative)
Applications
CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # BRA-11 (same as BRA-11G)
Format
Azide and BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Non-T, non-B CALLA positive ALL cell line REH (Leucocyte Workshop IV and V)
Reactivity Notes
Does not react with Rat or Rhesus Monkey.
Localization
Cell surface and cytoplasmic
Marker
B-Cell Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-33021).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for CD45RB Antibody (BRA-11 (same as BRA-11G)) - Azide and BSA Free
Western Blot: CD45RB Antibody (BRA-11 (same as BRA-11G))Azide and BSA Free [NBP2-34564]
Western Blot: CD45RB Antibody (BRA-11 (same as BRA-11G)) - Azide and BSA Free [NBP2-34564] - Western Blot Analysis of human Raji cell lysate using CD45RB Antibody (BRA-11 (same as BRA-11G)).![Immunohistochemistry-Paraffin: CD45RB Antibody (BRA-11 (same as BRA-11G)) - Azide and BSA Free [NBP2-34564]](https://resources.bio-techne.com/images/products/CD45RB-Antibody-BRA-11-same-as-BRA-11G-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-34564-img0003.jpg "Immunohistochemistry-Paraffin: CD45RB Antibody (BRA-11 (same as BRA-11G)) - Azide and BSA Free [NBP2-34564]")
Immunohistochemistry-Paraffin: CD45RB Antibody (BRA-11 (same as BRA-11G)) - Azide and BSA Free [NBP2-34564]
Immunohistochemistry-Paraffin: CD45RB Antibody (BRA-11 (same as BRA-11G)) - Azide and BSA Free [NBP2-34564] - Formalin-fixed, paraffin-embedded human tonsil stained with CD45RB Ab (BRA-11).![Flow Cytometry: CD45RB Antibody (BRA-11 (same as BRA-11G)) - Azide and BSA Free [NBP2-34564]](https://resources.bio-techne.com/images/products/CD45RB-Antibody-BRA-11-same-as-BRA-11G-Azide-and-BSA-Free-Flow-Cytometry-NBP2-34564-img0002.jpg "Flow Cytometry: CD45RB Antibody (BRA-11 (same as BRA-11G)) - Azide and BSA Free [NBP2-34564]")
Flow Cytometry: CD45RB Antibody (BRA-11 (same as BRA-11G)) - Azide and BSA Free [NBP2-34564]
Flow Cytometry: CD45RB Antibody (BRA-11 (same as BRA-11G)) - Azide and BSA Free [NBP2-34564] - Flow Cytometric Analysis of PFA-fixed Raji cells using CD45RB Antibody (BRA-11 (same as BRA-11G)).followed by Goat anti- Mouse- IgG-CF488 (Blue); Isotype Control (Red).Applications for CD45RB Antibody (BRA-11 (same as BRA-11G)) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
0.5-1ug/million cells
Immunocytochemistry/ Immunofluorescence
0.5-1ug/ml
Immunohistochemistry-Paraffin
0.5-1.0ug/ml
Western Blot
0.5-1.0ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: CD45RB
Long Name
CD45R, B isoform
Alternate Names
B220, CD45 antigen, CD45R, EC 3.1.3.48, L-CA, LY5, protein tyrosine phosphatase, receptor type, C, receptor-type tyrosine-protein phosphatase C, T200 glycoprotein, T200 leukocyte common antigen, T200receptor type, c polypeptide
Gene Symbol
PTPRC
Additional CD45RB Products
Product Documents for CD45RB Antibody (BRA-11 (same as BRA-11G)) - Azide and BSA Free
Product Specific Notices for CD45RB Antibody (BRA-11 (same as BRA-11G)) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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