CD47 Antibody (B6H12.2) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-31106

Novus Biologicals, part of Bio-Techne
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NBP2-31106
NBP2-31106-25ug
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

CyTOF-ready, Flow (Cell Surface), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # B6H12.2

Format

Azide and BSA Free

Concentration

1.0 mg/ml

View all CD47 Antibodies »

Product Specifications

Immunogen

This antibody was raised against CD47.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 30833751).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for CD47 Antibody (B6H12.2) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: CD47 Antibody (B6H12.2) - Azide and BSA Free [NBP2-31106]

Immunocytochemistry/ Immunofluorescence: CD47 Antibody (B6H12.2) - Azide and BSA Free [NBP2-31106]

Immunocytochemistry/Immunofluorescence: CD47 Antibody (B6H12.2) - Azide Free [NBP2-31106] - A431 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with CD47 Antibody [B6H12.2] (NBP2-31106) at 1 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Western Blot: CD47 Antibody (B6H12.2)Azide and BSA Free [NBP2-31106]

Western Blot: CD47 Antibody (B6H12.2)Azide and BSA Free [NBP2-31106]

Western Blot: CD47 Antibody (B6H12.2) - Azide Free [NBP2-31106] - Detection of CD47 expression on two hematological cancer cell lines, Jurkat and Raji. WB image submitted by a verified customer review.
Immunohistochemistry-Paraffin: CD47 Antibody (B6H12.2) - Azide and BSA Free [NBP2-31106]

Immunohistochemistry-Paraffin: CD47 Antibody (B6H12.2) - Azide and BSA Free [NBP2-31106]

Immunohistochemistry-Paraffin: CD47 Antibody (B6H12.2) - Azide Free [NBP2-31106] - Human breast cancer tissue stained with CD47 Antibody (B6H12.2). IHC-P image submitted by a verified customer review.
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Applications for CD47 Antibody (B6H12.2) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1 ug/mL

Immunohistochemistry-Paraffin

0.5 ug/mL

Western Blot

1:1000
Application Notes
This antibody is CyTOF ready.

Reviewed Applications

Read 3 reviews rated 4.7 using NBP2-31106 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CD47

CD47, originally named integrin-associated protein (IAP), is a 50-kDa protein containing five membrane spanning sequences and a short cytoplasmic tail. CD47 appears to be important in myeloid cell activation and migration across endothelial and epithelial monolayers. Anti-CD47 antibody has been shown to inhibit polymorphonuclear neutrophil (PMN) transmigration across cell monolayers and matrix. CD47 and its ligands, thrombospondin 1 (TSP-1) and SIRP-, are important regulators of dendritic cells. Recently, it has been shown that CD47 and TLR2 cross-talk to regulate PMN transmigration. The activation of TLR2/6 by MALP-2, a TLR2 specific agonist, potently inhibits human and murine PMN transmigration. The cross-talk between TLR2 and CD47 may be mediated through MYD88.

Alternate Names

CD47, IAP, MER6, OA3

Gene Symbol

CD47

OMIM

601028 (Human)

Additional CD47 Products

Product Documents for CD47 Antibody (B6H12.2) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for CD47 Antibody (B6H12.2) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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