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Key Product Details

Species Reactivity

Human, Cynomolgus Monkey, Rhesus Macaque

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, In vivo assay, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Human IgG1 kappa Clone # Campath-1H

Format

Azide and BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

Human lymphocytes

Specificity

Human CD52 antigen, also known as CAMPATH-1. CD52 is expressed at high density by lymphocytes, monocytes, eosinophils, thymocytes and macrophages. It is expressed by most lymphoid derived malignancies, although expression on myeloma cells is variable.

Clonality

Monoclonal

Host

Human

Isotype

IgG1 kappa

Scientific Data Images for CD52 Antibody (Campath-1H) - Azide and BSA Free

Immunohistochemistry: CD52 Antibody (Campath-1H) - Azide and BSA Free [NBP2-75896]

Immunohistochemistry: CD52 Antibody (Campath-1H) - Azide and BSA Free [NBP2-75896]

Immunohistochemistry: CD52 Antibody (Campath-1H) [NBP2-75896] - Anti-CD52 staining of formaldehyde fixed paraffin embedded rat thymus tissue, at 40x magnification. The human IgG1-chimeric version of Campath-1H (NBP2-75896) was used to stain samples at a concentration of 5 ug/ml.
Flow Cytometry: CD52 Antibody (Campath-1H) - Azide and BSA Free [NBP2-75896]

Flow Cytometry: CD52 Antibody (Campath-1H) - Azide and BSA Free [NBP2-75896]

Flow Cytometry: CD52 Antibody (Campath-1H) [NBP2-75896] - Flow-cytometry using anti-CD52 antibody Campath-1H (NBP2-75896). Cynomolgus monkey lymphocytes were stained with an isotype control (NBP2-52638, panel A) or the rabbit-chimeric version of Campath-1H (NBP2-75189, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.
Immunohistochemistry: CD52 Antibody (Campath-1H) - Azide and BSA Free [NBP2-75896]

Immunohistochemistry: CD52 Antibody (Campath-1H) - Azide and BSA Free [NBP2-75896]

Immunohistochemistry: CD52 Antibody (Campath-1H) [NBP2-75896] - Formalin fixed human thyroid cancer slices were were stained with a NBP2-75896 at 5 ug/ml.

Applications for CD52 Antibody (Campath-1H) - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

In vivo assay

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
NOT FOR THERAPEUTIC USE - This is a research-grade biosimilar.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.

Background: CD52

Human CD52 is a glycosylphosphatidylinositol (GPI)anchored antigen expressed in a number of different tissues.

Alternate Names

CAMPATH-1, CD52, CDw52, He5, HEL-S-171mP, biosimilar antibody, biosimilars

Gene Symbol

CD52

Additional CD52 Products

Product Documents for CD52 Antibody (Campath-1H) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CD52 Antibody (Campath-1H) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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