CD52 Antibody (HI186) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB500-311

Clone HI186 was used by HLDA to establish CD designation.
Novus Biologicals, part of Bio-Techne
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NB500-311
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Key Product Details

Species Reactivity

Validated:

Human

Applications

CyTOF-ready, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # HI186

Format

BSA Free

Concentration

1.0 mg/ml

View all CD52 Antibodies »

Product Summary for CD52 Antibody (HI186) - BSA Free

Immunogen

Human tonsil (NM_001803.2).

Specificity

The antibody HI186 reacts with CD52 (CAMPATH-1), a 21-28 kDa glycoprotein containing a large N-linked carbohydrate moiety; mature CD52 molecule is actually much smaller (approx. 8-9 kDa). CD52 is expressed at high levels on lymphocytes, monocytes/macrophages and in male reproductive tract. HLDA VI; WS Code BP 523 HLDA VI; WS Code T 6T-057

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

28 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CD52 Antibody (HI186) - BSA Free

Flow Cytometry: CD52 Antibody (HI186) - BSA Free [NB500-311]

Flow Cytometry: CD52 Antibody (HI186) - BSA Free [NB500-311]

Flow Cytometry: CD52 Antibody (HI186) [NB500-311] - Flow cytometry analysis of CD52 in patients suffering with Acute Lymphoblastic Leukemia (anti-human CD52 (HI186); detection by Goat anti-mouse IgG2b PE). Fig. 1A CD52 positive cALL (gate leukemic cells).
Flow Cytometry: CD52 Antibody (HI186) - BSA Free [NB500-311]

Flow Cytometry: CD52 Antibody (HI186) - BSA Free [NB500-311]

Flow Cytometry: CD52 Antibody (HI186) [NB500-311] - Fig.1B CD52 negative pro T ALL (gate leukemic cells)

Applications for CD52 Antibody (HI186) - BSA Free

Application
Recommended Usage

Flow Cytometry

2 ug/ml

Immunohistochemistry

1:200

Immunohistochemistry-Paraffin

1:200
Application Notes
Purity is > 95% (by SDS-PAGE). This antibody is CyTOF ready.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Format

BSA Free

Preservative

0.9% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CD52

CD52 (CAMPATH-1, HE5) is a highly glycosylated GPI-anchored 21-28 kDa glycopeptide which is present at high levels on lymphocytes, macrophages, epithelial cells of male reproductive tract and mature sperm. Its 12-amino acid beckbone carries a complex N-linked carbohydrate moiety, which differs between sperm and leukocyte CD52, as well as the GPI anchor does. CD52 can be acquired by sperm cells from seminal plasma, where it is released by epithelial cells. Although CD52 is not an essential T-cell costimulator, its triggering results in activation of normal human T cells. CD52 is a very good target for antibody/complement-mediated cell lysis.

Alternate Names

CAMPATH-1, CD52, CDw52, He5, HEL-S-171mP

Entrez Gene IDs

1043 (Human)

Gene Symbol

CD52

UniProt

P31358

Additional CD52 Products

Product Documents for CD52 Antibody (HI186) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CD52 Antibody (HI186) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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