CD52 Antibody (YTH 34.5-G2b (Campath-1G)) - Chimeric - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-52657
Recombinant Monoclonal Antibody. Clone YTH 34.5-G2b (Campath-1G) was used by HLDA to establish CD designation.
![Western Blot: CD52 Antibody (YTH 34.5-G2b (Campath-1G))ChimericAzide and BSA Free [NBP2-52657]](https://resources.bio-techne.com/images/products/CD52-Antibody-YTH-34-5-G2b-Campath-1G-Chimeric-Western-Blot-NBP2-52657-img0002.jpg "Western Blot: CD52 Antibody (YTH 34.5-G2b (Campath-1G))ChimericAzide and BSA Free [NBP2-52657]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Cynomolgus Monkey, Rhesus Macaque
Applications
CyTOF-ready, ELISA, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, In vivo assay, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Kappa Clone # YTH 34.5-G2b (Campath-1G)
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Human peripheral mononuclear cells enriched for T-cells.
Specificity
Human CD52 antigen, also known as CAMPATH-1. CD52 is expressed at high density by lymphocytes, monocytes, eosinophils, thymocytes and macrophages. It is expressed by most lymphoid derived malignancies, although expression on myeloma cells is variable.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Kappa
Scientific Data Images
Western Blot: CD52 Antibody (YTH 34.5-G2b (Campath-1G))ChimericAzide and BSA Free [NBP2-52657]
Western Blot: CD52 Antibody (YTH 34.5-G2b (Campath-1G)) - Chimeric [NBP2-52657] - Western Blot using anti-CD52 antibody YTH 34.5-G2b (Campath-1G). Mouse spleen samples were resolved on a 15% SDS PAGE gel and blots probed with the rat IgG1-chimeric version of YTH 34.5-G2b at 1.5 ug/ml before detection using an anti-rat secondary antibody. Protein was detected by chemiluminescence. The expected unmodified running size for CD52 is ~8kDa, but this protein is extensively glycosylated and exhibits running sizes of ~11-25kDa.![Flow Cytometry: CD52 Antibody (YTH 34.5-G2b (Campath-1G)) - Chimeric - Azide and BSA Free [NBP2-52657]](https://resources.bio-techne.com/images/products/CD52-Antibody-YTH-34-5-G2b-Campath-1G-Chimeric-Flow-Cytometry-NBP2-52657-img0001.jpg "Flow Cytometry: CD52 Antibody (YTH 34.5-G2b (Campath-1G)) - Chimeric - Azide and BSA Free [NBP2-52657]")
Flow Cytometry: CD52 Antibody (YTH 34.5-G2b (Campath-1G)) - Chimeric - Azide and BSA Free [NBP2-52657]
Flow Cytometry: CD52 Antibody (YTH 34.5-G2b (Campath-1G)) - Chimeric [NBP2-52657] - Flow-cytometry using the anti-CD52 research biosimilar antibody Campath-1G (YTH 34.5-G2b) Human lymphocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of Campath-1G / YTH 34.5-G2b (NBP2-52657, panel B) at a concentration of 0.25 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.Applications
Application
Recommended Usage
ELISA
1:100 - 1:2000
Flow Cytometry
1:10 - 1:1000
Immunohistochemistry-Frozen
1:10 - 1:500
Immunohistochemistry-Paraffin
1:10 - 1:500
Western Blot
1:100 - 1:2000
Application Notes
This antibody is Cytof ready. This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2b format, for improved compatibility with existing reagents, assays and techniques.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
0.02% Proclin 300
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
Background: CD52
Alternate Names
CAMPATH-1, CD52, CDw52, He5, HEL-S-171mP, biosimilar antibody, biosimilars
Gene Symbol
CD52
Additional CD52 Products
Product Specific Notices
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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